BIOCHEMISTRY AND MOLECULAR BIOLOGY

Maria Caterina TURCO BIOCHEMISTRY AND MOLECULAR BIOLOGY

1060100050
DEPARTMENT OF MEDICINE, SURGERY AND DENTISTRY "SALERNITANA MEDICAL SCHOOL"
EQF7
MEDICINE AND SURGERY
2023/2024

OBBLIGATORIO
YEAR OF COURSE 2
YEAR OF DIDACTIC SYSTEM 2016
FULL ACADEMIC YEAR
CFUHOURSACTIVITY
1BIOCHIMICA E BIOLOGIA MOLECOLARE - MOD. BIOCHIMICA
10120LESSONS
2BIOCHIMICA E BIOLOGIA MOLECOLARE - MOD. BIOLOGIA MOLECOLARE
560LESSONS
Objectives
THIS COURSE IS DESIGNED TO PROVIDE STUDENTS WITH: FUNDAMENTALS OF BIOMOLECULES STRUCTURES AND FUNCTIONS. FUNDAMENTALS OF MAIN METABOLIC PATHWAYS. FURTHERMORE, THE STUDENT WILL LEARN THE FUNDAMENTAL MOLECULAR MECHANISMS RELATED TO THE PROPAGATION AND MAINTENANCE OF GENETIC INFORMATION; WILL KNOW THE MOLECULAR MECHANISMS OF GENE EXPRESSION AND ITS REGULATIONS; WILL BE ABLE TO DESCRIBE AND SELECT APPROPRIATE TECHNOLOGIES FOR THE ANALYSIS OF BIOMOLECULAR MECHANISMS AND THE IDENTIFICATION OF MOLECULAR ORIGIN OF THE DISEASE, WILL KNOW THE GENERAL PROCESSES THROUGH WHICH GENETIC INFORMATION IS TRANSMITTED AND EXPRESSED.
IN AGREEMENT WITH THE DUBLIN DESCRIPTORS, AT THE END OF THE COURSE THE STUDENT WILL DEMONSTRATE:
1.TO HAVE REACHED A BASIC KNOWLEDGE AND UNDERSTANDING OF BIOCHEMISTRY THAT ALLOWS HIM TO SET UP THE ANALYSIS OF THE CORRELATIONS BETWEEN THE STRUCTURE OF BIOMOLECULES AND THEIR FUNCTIONS IN PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS;
2.THE ABILITY TO ANALYZE, DISCUSS AND PERFORM SIMPLE CORRELATIONS OF DATA OR ASSUMPTIONS;
3.BEING ABLE EVALUATE INDEPENDENTLY ANY DIFFERENT OPINIONS EXIST IN CERTAIN TOPICS TREATED IN THE COURSE;
4.BEING ABLE TO PROPERLY EXPOSE IN ITALIAN AND WITH PROPERTIES OF LANGUAGE KNOWLEDGE ACQUIRED;
5.KNOWING HOW TO COMMUNICATE WITH THEIR PEERS IN A CRITICAL MANNER THE THEMES STUDIED AND TO SUPPORT INTERDISCIPLINARY ARGUMENTS.
Prerequisites
IN ORDER TO UNDERSTAND AND KNOW HOW TO APPLY THE TOPICS DISCUSSED IN TO THE COURSE STUDENTS MUST HAVE PASSED THE CHEMISTRY AND PROPEDEUTIC BIOCHEMISTRY AND APPLIED BIOLOGY
Contents
THE COURSE IS SUPPLEMENTED BY TWO MODULES: MODULE A BIOCHEMISTRY (10 CFU) AND MODULE B MOLECULAR BIOLOGY (5CFU).
THE TOPICS COVERED IN THE BIOCHEMISTRY MODULE ARE: AMINO ACIDS AND PEPTIDES: CLASSIFICATION BASED ON THEIR CHEMICAL-PHYSICAL PROPERTIES. PEPTIDIC BOND. LEVELS OF STRUCTURAL PROTEIN ORGANIZATION: PRIMARY STRUCTURE, SECONDARY STRUCTURE; TERTIARY STRUCTURE AND ORGANIZATION IN DOMAIN; QUATERNARY STRUCTURE, PROTEIN-PROTEIN INTERACTIONS. FIBROSE AND GLOBULAR PROTEINS.
ENZYMES: GENERAL PROPERTIES AND CLASSIFICATION; MECHANISM OF ACTION OF ENZYMES KINETICS OF ENZYMATIC REACTIONS: EQUATION OF MICHAELIS-MENTEN. DOUBLE-RECIPROCAL PLOTS. FACTORS THAT MODULATE THE SPEED OF AN ENZYMATIC REACTION. ENZYMATIC INHIBITION: REVERSIBLE AND IRREVERSIBLE INHIBITION. COMPETITIVE, INCOMPETITIVE AND NON –COMPETITIVE INHIBITORS.
COENZYMES AND PROSTHETIC GROUPS. COENZYMES AND VITAMINS, STRUCTURE AND FUNCTION. REGULATION OF ENZYMATIC ACTIVITY: ALLOSTERIC REGULATION; POST-TRASLATIONAL MODIFICATIONS. ZYMOGENS, ISOENZYMES AND MULTIENZYME COMPLEXES.
GENERAL CONCEPTS AND DESIGNS OF METABOLISM. CATABOLIC, ANABOLIC AND ANFIBOLICHE PATHWAYS AND THEIR REGULATION. PRINCIPLES OF BIOENERGY AND THERMODYNAMICS: THE ATP CYCLE. OXIDATIVE PHOSPHORYLATION.
CARBOHYDRATES: CHEMICAL-PHYSICAL PROPERTIES AND CLASSIFICATION.
CARBOHYDRATES METABOLISM: GLYCOLYSIS, ALCOHOLIC FERMENTATION AND LACTIC FERMENTATION. GLYCOLYSIS REGULATION. PYRUVATE OXIDATIVE DECARBOXYLATION AND ITS REGULATION. GLYCOGEN: GLYCOGENOLYSIS AND GLICOGENOSYNTHESIS AND ITS REGULATION. PENTOSE PHOSPHATE AND GLUCONEOGENESIS PATHWAY. CORI CYCLE.
LIPIDS: BIOLOGICAL MEMBRANES. LIPIDS METABOLISM: CLASSIFICATION OF LIPIDS. OXIDATION OF FATTY ACIDS FATTY ACIDS OF PAIR OR UNPAIR NUMBER OF CARBON ATOMS. OXIDATION OF UNSATURATED FATTY ACIDS. ENERGY BALANCE OF OXIDATION OF SATURATED AND UNSATURATED FATTY ACIDS.
FORMATION AND OXIDATION OF KETONE BODIES. BIOSYNTHESIS OF SATURATED FATTY ACIDS AND REGULATION.
AMINO ACIDS CATABOLISM: TRANSAMINATION AND OXIDATIVE DEAMINATION. FATE OF THE CARBONACEOUS SKELETON OF AMINO ACIDS. THE UREA CYCLE.
KREBS CYCLE. METABOLIC REGULATION OF THE CYCLE. BIOSYNTHETIC ROLE OF THE CYCLE: ANAPLEROTICHE REACTIONS.
THE TOPICS COVERED IN THE MOLECULAR BIOLOGY MODULE ARE:
STRUCTURE AND ORGANIZATION OF GENETIC MATERIAL IN PROKARYOTES, VIRUSES AND EUKARYOTES; STRUCTURE AND TOPOLOGY OF DNA; STRUCTURE OF RNA; CHROMATIN; HISTONES AND NUCLEOSOMES; REGULATION OF CHROMATIN STRUCTURE; EPIGENETICS.
REPLICATION AND REPAIR OF DNA DAMAGE: MECHANISM OF ACTION OF DNA POLYMERASES, STAGE OF START AND TERMINATION OF REPLICATION, TELOMERES AND TELOMERASE; REPLICATION ERRORS AND THEIR REPAIR; DIFFERENT TYPES OF MUTATIONS.
TRANSCRIPTION AND POST-TRANSCRIPTIONAL MODIFICATIONS, STRUCTURE AND FUNCTION OF RNA POLYMERASES, PROMOTERS OF RNA POLYMERASE I, II AND III; GENERAL TRANSCRIPTION FACTORS, THE MEDIATOR COMPLEX; MESSENGER RNA PROCESSING: CAPPING, SPLICING AND POLYADENYLATION; THE SPLICEOSOME, ALTERNATIVE SPLICING.
TRANSLATION: THE GENETIC CODE; STRUCTURE AND FUNCTION OF TRNA, AMINOACYL-TRNA SYNTHETASES, THE RIBOSOME, INITIATION, ELONGATION AND TERMINATION OF TRANSLATION.
REGULATION OF GENE EXPRESSION: TRANSCRIPTIONAL ACTIVATORS; SIGNAL INTEGRATION AND COMBINATORIAL CONTROL, SIGNAL TRANSDUCTION AND CONTROL OF TRANSCRIPTIONAL REGULATORS; GENE SILENCING, RNA INTERFERENCE; SYNTHESIS AND FUNCTION OF MIRNAS.
MOLECULAR BIOLOGY TECHNOLOGY AND GENE EXPRESSION ANALYSIS: PURIFICATION OF BIOLOGICVAL MACROMOLECULES; ELECTROPHORESIS, RESTRICTION ENZYMES; EMSA; FOOTPRINTING; PCR, RT-PCR, DNA SEQUENCING, MOLECULAR DIAGNOSTICS AND CLINICAL IMPLICATIONS; HIGH THROUGHPUT TECHNOLOGIES.
Teaching Methods
THE COURSE IS DIVIDED INTO TWO MODULES AND PROVIDES 180 HOURS OF ASSISTED LEARNING (15 CFU) ALLOCATED BETWEEN THE TEACHING HOURS IN THE FORM OF CLASSROOM LECTURES AND HOURS OF SUPPLEMENTARY ACTIVITIES (ADI) CONSIST OF SEMINARS, LABORATORY EXERCISES, PRACTICE EXAMS, CORRECTIONS, STUDY GROUPS FOR THE ANALYSIS AND ELABORATION OF SCIENTIFIC PUBLICATIONS.
120 HOURS (10CFU) ARE OCCUPIED BY THE BIOCHEMISTRY MODULE, AND 60 HOURS (6 CFUS) FROM THE MOLECULAR BIOLOGY MODULE. IN THE BIOCHEMISTRY MODULE, 80 HOURS OF LESSON ARE DELIVERED IN THE CLASSROOM WITH THE AID OF PROJECTIONS AND IN THE 40 HOURS, THERE ARE GUIDED LABORATORY EXERCISES, PRACTICE EXAMS AND CORRECTIONS. IN THE MOLECULAR BIOLOGY MODULE, 40 HOURS ARE DELIVERED AS FRONTAL LECTURES IN THE CLASSROOM WITH THE AID OF PROJECTIONS AND 20 HOURS OF SUPPLEMENTARY DIDACTICS DURING WHICH THE STUDENTS, SUBDIVIDED INTO STUDY GROUPS, WILL UNDERTAKE THE DEEPENING OF REGULATORY PROCESSES AND MOLECULAR MECHANISMS INVOLVED IN THE DEVELOPMENT OF HUMAN PATHOLOGIES THROUGH THE ANALYSIS AND ELABORATION OF SCIENTIFIC PUBLICATIONS SELECTED BY THE TEACHER.
THE FREQUENCY OF CLASSROOM LESSONS AND LABORATORY EXERCISES IS COMPULSORY. TO QUALIFY FOR THE FINAL EXAM, THE STUDENT MUST HAVE ATTENDED AT LEAST 70% OF THE TUITION HOURS. THE ATTENDANCE VERIFICATION MODE IS ANNOUNCED BY THE TEACHER AT THE BEGINNING OF THE LESSONS.
Verification of learning
THE LEVEL OF ACHIEVEMENT OF THE OBJECTIVES OF TEACHING IS CERTIFIED BY PASSING AN EXAM WITH EVALUATION OF THIRTY. VERIFICATION INVOLVES A WRITTEN TEST PASSED WHICH THE STUDENT WILL BE ABLE TO TAKE THE ORAL TEST THAT TAKES PLACE ON THE SAME DAY.
THE WRITTEN TEST CONSISTS OF 30 MULTIPLE CHOICE QUESTIONS ON TOPICS OF THE TEACHING PROGRAM TEST. THE TEST TAKES PLACE PRIOR TO THE ORAL TEST AND IS PASSED WITH THE ACHIEVEMENT OF THE PREDETERMINED MINIMUM SCORE OF 18/30. THE WRITTEN TEST WILL NORMALLY HAVE A DURATION OF 30 MINUTES AND IS DESIGNED TO TEST THE ABILITY TO PROPERLY APPLY THE THEORETICAL KNOWLEDGE AND THE PROPOSALS ISSUES UNDERSTANDING.
THE ORAL TEST IS INTENDED TO ASCERTAIN THE LEVEL OF KNOWLEDGE OF THEORETICAL CONTENTS IN THE EVENT OF ERRORS IN THE WRITTEN TEST; THE LEVEL OF EXPERTISE ACHIEVED IN THE EXHIBITION SUCH AS LANGUAGE PROPERTY, LOGICAL - DEDUCTIVE AND SYNTHESIS SKILLS. THE FINAL VOTE COMES FROM THE TWO TRIALS. THE MINIMUM VOTE FOR PASSING THE EXAM IS 18/30. PRAISE MAY BE ATTRIBUTED TO STUDENTS WHO DEMONSTRATE THAT THEY ARE ABLE TO APPLY THEIR OWN KNOWLEDGE AND SKILLS ACQUIRED IN CONTEXTS OTHER THAN THOSE PROPOSED IN LESSONS.

Texts
BAYNES AND DOMINICZAK, BIOCHIMICA MEDICA, ED. ELSEVIER
LEHNINGER, NELSON & COX, PRINCIPI DI BIOCHIMICA, ED. ZANICHELLI
MATHEWS, VAN HOLD, AHEREN, BIOCHIMICA, ED. AMBROSIANA
STRYER, BIOCHIMICA, ED. ZANICHELLI
DEVLIN BIOCHIMICA CON ASPETTI CLINICI ED EDISES
CAMPBELL & FARRELL, BIOCHIMICA, ED. EDISES
L.A. ALLISON, FONDAMENTI DI BIOLOGIA MOLECOLARE II EDIZIONE. CASA EDITRICE ZANICHELLI
J.D. WATSON ET AL, BIOLOGIA MOLECOLARE DEL GENE, OTTAVA EDIZIONE CASA ED. ZANICHELLI
LEWIN ET AL., CELLULE, CASA ED. ZANICHELLI
ALBERTS ET AL., “BIOLOGIA MOLECOLARE DELLA CELLULA”, V EDIZIONE, CASA ED. ZANICHELLI
EDUCATIONAL MATERIAL SUPPLIED BY THE TEACHERS
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