BASIC BIOCHEMISTRY BIOLOGY AND MICROBIOLOGY

Rosanna MARTINELLI BASIC BIOCHEMISTRY BIOLOGY AND MICROBIOLOGY

1012100001
DIPARTIMENTO DI MEDICINA, CHIRURGIA E ODONTOIATRIA "SCUOLA MEDICA SALERNITANA"
EQF6
NURSING
2016/2017

OBBLIGATORIO
YEAR OF COURSE 1
YEAR OF DIDACTIC SYSTEM 2013
PRIMO SEMESTRE
CFUHOURSACTIVITY
1NOZIONI DI BIOCHIMICA BIOLOGIA E MICROBIOLOGIA - MOD BIOCHIMICA
224LESSONS
2NOZIONI DI BIOCHIMICA BIOLOGIA E MICROBIOLOGIA - MOD MICROBIOLOGIA
112LESSONS
3NOZIONI DI BIOCHIMICA BIOLOGIA E MICROBIOLOGIA - MOD BIOLOGIA APPLICATA
112LESSONS


Objectives
AT THE END OF THE COURSE THE STUDENT WILL KNOW THE STRUCTURE OF THE MAIN MOLECULES OF BIOCHEMICAL INTEREST AND HAVE UNDERSTOOD THE BASIC PRINCIPLES OF BIOENERGETICS FOR THE STUDY OF METABOLISM. MOREOVER IT SHOULD KNOW THE MAIN TECHNOLOGIES USED IN MOLECULAR DIAGNOSTICS.
Prerequisites
NONE
Contents
ELEMENTS OF CHEMISTRY: PHYSICAL QUANTITIES AND UNITS OF MEASURE; MULTIPLES AND SUBMULTIPLES; PROPERTIES OF MATTER, HOMOGENEOUS AND HETEROGENEOUS SYSTEMS; THE CHEMICAL BONDS; ELECTRONEGATIVITY; IONIC BOND; COVALENT BOND; HYDROGEN BOND; STATES OF MATTER; CHEMICAL REACTIONS, CHEMICAL EQUILIBRIUM; SOLUTIONS; SOLUBILITY; COLLIGATIVE PROPERTIES OF SOLUTIONS; THE CONCENTRATIONS OF THE SOLUTIONS; THE WATER; PH AND BUFFER SOLUTIONS.

THE CELL UNIT OF LIFE: THE AIM OF BIOCHEMISTRY; PROKARYOTIC AND EUKARYOTIC CELLS; THE MAIN BIOCHEMICAL COMPONENTS OF THE HUMAN BODY; CELLULAR LOCALIZATION OF METABOLIC PROCESSES.

AMINO ACIDS AND PROTEINS: STRUCTURAL PROPERTIES AND CLASSIFICATION OF AMINO ACIDS; THE PEPTIDE BOND; PROTEIN STRUCTURE: PRIMARY, SECONDARY, TERTIARY AND QUATERNARY; MYOGLOBIN AND HEMOGLOBIN.

ENZYMES: GENERAL PROPERTIES OF ENZYMES; ENZYME KINETICS; ENZYME INHIBITION; MULTISUBSTRATE REACTIONS; REGULATION OF ENZYME ACTIVITY; CLINICAL SIGNIFICANCE OF ENZYMES.

NUCLEOTIDE AND NUCLEIC ACIDS: STRUCTURE OF NUCLEIC ACIDS, DNA; RNA; MOLECULAR DIAGNOSTICS; MUTATIONS; PURIFICATION OF NUCLEIC ACIDS; RESTRICTION ENZYMES; PCR; ELECTROPHORESIS.

BIOENERGETICS AND METABOLISM: BIOENERGETICS AND TYPES OF BIOCHEMICAL REACTIONS; PRINCIPLES OF METABOLIC REGULATION

GLYCOLYSIS: THE CHEMISTRY OF CARBOHYDRATES; THE DIGESTION OF CARBOHYDRATES; THE GLYCOLYTIC PATHWAY.

KREBS CYCLE: DECARBOXYLATION REACTIONS; OVERVIEW OF THE KREBS CYCLE; YIELD OF ATP; REGULATION OF THE OXIDATION OF PYRUVATE AND ACETYL-COA; CLINICAL ASPECTS OF OXIDATIVE METABOLISM.

AMINO ACID OXIDATION: METABOLIC FATE OF AMINO GROUPS; NITROGEN EXCRETION AND UREA CYCLE .
Teaching Methods
LECTURES
Verification of learning
WRITTEN AND ORAL EXAM
Texts
SUGGESTED TEXT: INTRODUZIONE ALLA BIOCHIMICA DI LEHNINGER. NELSON D.L., COX MM. ZANICHELLI
TEACHING MATERIALS: SLIDES OF THE LECTURES.
More Information
NONE
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