ALBINO CARRIZZO | ELEMENTS OF BIOCHEMISTRY AND LABORATORY MEDICINE

cod. 0612800006

ELEMENTS OF BIOCHEMISTRY AND LABORATORY MEDICINE

	0612800006
	DEPARTMENT OF INFORMATION AND ELECTRICAL ENGINEERING AND APPLIED MATHEMATICS
	EQF6
	INFORMATION ENGINEERING FOR DIGITAL MEDICINE
	2024/2025

PERCORSO COMUNE

	OBBLIGATORIO
	YEAR OF COURSE 1
	YEAR OF DIDACTIC SYSTEM 2022
	SPRING SEMESTER

TEACHING UNITS

	SSD	CFU	HOURS	ACTIVITY	TYPE OF ACTIVITY
1	ELEMENTI DI BIOCHIMICA E MEDICINA DI LABORATORIO
	BIO/10	3	24	LESSONS	SUPPLEMENTARY COMPULSORY SUBJECTS
2	ELEMENTI DI BIOCHIMICA E MEDICINA DI LABORATORIO
	MED/46	2	16	LESSONS	SUPPLEMENTARY COMPULSORY SUBJECTS
	MED/46	1	8	EXERCISES	SUPPLEMENTARY COMPULSORY SUBJECTS

PROFESSORS

	ALESSANDRA ROSATI1 T Curriculum
	ALBINO CARRIZZO2 Curriculum

	Objectives
	THE MAIN OBJECTIVES OF THIS COURSE ARE: TO LEARN THE BASIC MECHANISMS OF CHEMICAL AND METABOLIC TRANSFORMATION, THE MOLECULAR EVENTS UNDERLYING HUMAN PHYSIOLOGY AND PATHOLOGY, AND THE CHEMICAL, BIOCHEMICAL AND METABOLIC CHANGES IN CLINICAL PATHOLOGY. KNOWLEDGE AND UNDERSTANDING THE FUNDAMENTAL ASPECTS OF HUMAN BIOCHEMISTRY, ARE PARTICULARLY RELATED TO THE INTEGRATED METABOLISM OF GLUCOSE, AMINO ACIDS, AND LIPIDS AND THEIR RELATIONSHIP TO METABOLIC PATHOLOGY. THE STRUCTURAL FEATURES OF THE MAJOR MACROMOLECULES SUCH AS PROTEINS, CARBOHYDRATES, LIPIDS, AND NUCLEIC ACIDS, AND THE STRATEGIES USED TO REGULATE THEIR FUNCTION. BIOMOLECULAR TRANSFORMATIONS AND ASSOCIATED ENERGY CHANGES. THE MAIN TECHNIQUES FOR BIOCHEMICAL ANALYSIS. NUCLEIC ACID (DNA, RNA) AND TRANSMISSION OF GENETIC INFORMATION. MAJOR BIOLOGICAL DATABASES: GENOMICS AND PROTEOMICS DATABASES. ELEMENTS OF MEDICAL GENETICS AND RELATED DIAGNOSTIC CLINICAL ASPECTS. APPLIED KNOWLEDGE AND UNDERSTANDING RECOGNIZE THE BIOMOLECULES PROPERTIES ASSOCIATED WITH THE PRESENCE OF SPECIFIC FUNCTIONAL GROUPS. APPLY THE REACTION MECHANISMS OF ORGANIC COMPOUNDS TO UNDERSTAND BIOCHEMICAL REACTIONS. DESCRIBE AND SELECT THE APPROPRIATE TECHNIQUE FOR ANALYZING BIOMOLECULAR MECHANISMS. RECOGNIZE THE MOLECULAR ORIGIN OF PATHOLOGIES. MAP THE BIOLOGICAL PROBLEM OF INTEREST TO DATA PROCESSING AND ANALYTICAL OPERATIONS. USE OF GENE ANALYSIS METHODS. USE OF BIOLOGICAL DATABASE. INTERPRETATION AND USE OF LABORATORY DATA.

THE MAIN OBJECTIVES OF THIS COURSE ARE: TO LEARN THE BASIC MECHANISMS OF CHEMICAL AND METABOLIC TRANSFORMATION, THE MOLECULAR EVENTS UNDERLYING HUMAN PHYSIOLOGY AND PATHOLOGY, AND THE CHEMICAL, BIOCHEMICAL AND METABOLIC CHANGES IN CLINICAL PATHOLOGY.
KNOWLEDGE AND UNDERSTANDING
THE FUNDAMENTAL ASPECTS OF HUMAN BIOCHEMISTRY, ARE PARTICULARLY RELATED TO THE INTEGRATED METABOLISM OF GLUCOSE, AMINO ACIDS, AND LIPIDS AND THEIR RELATIONSHIP TO METABOLIC PATHOLOGY.
THE STRUCTURAL FEATURES OF THE MAJOR MACROMOLECULES SUCH AS PROTEINS, CARBOHYDRATES, LIPIDS, AND NUCLEIC ACIDS, AND THE STRATEGIES USED TO REGULATE THEIR FUNCTION. BIOMOLECULAR TRANSFORMATIONS AND ASSOCIATED ENERGY CHANGES. THE MAIN TECHNIQUES FOR BIOCHEMICAL ANALYSIS. NUCLEIC ACID (DNA, RNA) AND TRANSMISSION OF GENETIC INFORMATION. MAJOR BIOLOGICAL DATABASES: GENOMICS AND PROTEOMICS DATABASES. ELEMENTS OF MEDICAL GENETICS AND RELATED DIAGNOSTIC CLINICAL ASPECTS.
APPLIED KNOWLEDGE AND UNDERSTANDING
RECOGNIZE THE BIOMOLECULES PROPERTIES ASSOCIATED WITH THE PRESENCE OF SPECIFIC FUNCTIONAL GROUPS. APPLY THE REACTION MECHANISMS OF ORGANIC COMPOUNDS TO UNDERSTAND BIOCHEMICAL REACTIONS. DESCRIBE AND SELECT THE APPROPRIATE TECHNIQUE FOR ANALYZING BIOMOLECULAR MECHANISMS. RECOGNIZE THE MOLECULAR ORIGIN OF PATHOLOGIES. MAP THE BIOLOGICAL PROBLEM OF INTEREST TO DATA PROCESSING AND ANALYTICAL OPERATIONS. USE OF GENE ANALYSIS METHODS. USE OF BIOLOGICAL DATABASE. INTERPRETATION AND USE OF LABORATORY DATA.

	Prerequisites
	PREREQUISITE: CHEMISTRY

	Contents
	Didactic Unit 1: STRUCTURE AND PROPERTIES OF THE MAIN MACROMOLECULES OF BIOLOGICAL INTEREST (LESSON /PRACTICE / LABORATORY HOURS 10/2/0) - 1 (2 HOURS Lesson): Introduction to biological macromolecules. Proteins as amino acid polymers; nucleic acids as nucleotide polymers; polysaccharides and lipids -2 (2 HOURS Lecture) Elements of cellular architecture. Membranes, organelles and their functions, cell compartments, ribosomes. Cellular signaling and cellular response. - 3 (2 HOURS Lesson): Protein structure: peptide bond, secondary and higher level protein structures. Structure-activity relationships. - 4 (2 HOURS Lesson): Enzymes: catalysis, enzymatic kinetics (basic), role of catalysis in metabolism - 5 (2 HOURS Lesson): Nucleic acids functionalities in protein synthesis. DNA and RNA structures. Basic concepts in transcription and translation. Chromatin structure. - 6 (2 HOURS Practice): Use of sequences database (nucleic acids, proteins). Sequence alignment tools. KNOWLEDGE AND UNDERSTANDING: FUNCTIONS OF MOLECULES OF BIOLOGICAL INTEREST APPLIED KNOWLEDGE AND UNDERSTANDING: ABILITY TO CORRELATE MAJOR BIOMOLECULES STRUCTURES WITH THEIR FUNCTIONS IN PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS. Unità didattica 2: CELLULAR METABOLISM (LESSON /PRACTICE / LABORATORY HOURS 10/2/0) - 1 (2 HOURS Lesson) Nutritional principles and their assimilation. Macromolecules as a source of energy. 2 (2 HOURS Lesson) Metabolism and energy; ATP, energy transfer, coupling of endo / exo-ergonic reactions. - 3 (2 HOURS Lesson): carbohydrate metabolism. glycolysis and fermentation. glycogen metabolism - 4 (2 HOURS Lesson): metabolism of lipids and amino acids. - 5 (2 HOURS Lesson): cycle of tricarboxylic acids and energy production through oxidative phosphorylation. electron transport chain. - 6 (2 HOURS Exercise): Connection of the concepts covered to the functionality of the cells; examples of pathologies related to metabolism disregulation. KNOWLEDGE AND UNDERSTANDING: KNOWING THE MAIN BIOCHEMICAL TRANSFORMATIONS AND CONNECTING THEM TO THE MAIN CELLULAR ACTIVITIES. APPLIED KNOWLEDGE AND UNDERSTANDING APPLIED: IDENTIFY THE MEANING OF BIOMARKER. Learning Unit 3: FUNDAMENTALS OF LABORATORY MEDICINE. (HOURS LECTURE/EXERCISE/LABORATORY 8/2/0) - 1 (2 HOURS Lecture): Introduction to laboratory medicine, the analytical method, organization of the biomedical laboratory, objectives of laboratory medicine - 2 (2 HOURS Lecture): Instrumental and biochemical data collection, differentiation of biological specimens and sources of errors - 3 (2 HOURS Lecture): The main techniques of laboratory medicine, the concept of biomarkers and genetic predisposition to disease -4 (2 HOURS Lecture): the concept of new devices that can be used in medicine, experimental medical devices and devices already approved by the National Institute of Health 5 (2 HOURS Exercise): The use of main scientific search engines and the define the state of the art of the disease of interest KNOWLEDGE AND UNDERSTANDING SKILLS: KNOW THE ROLE OF LABORATORY MEDICINE IN MONITORING HEALTH AND DISEASE STATUS, UNDERSTAND MEASURABLE PARAMETERS IN BIOLOGICAL SAMPLES APPLIED KNOWLEDGE AND CAPABILITY OF UNDERSTANDING: SELECT THE BEST MARKER OF PATHOLOGY and Hypothesize THE DEVELOPMENT OF NEW POSSIBLE TOOLS Learning Unit 4: THE ROLE OF LABORATORY MEDICINE IN THE IDENTIFICATION AND FOLLOW-UP OF PATHOLOGIES. (HOURS LECTURE/EXERCISE/LABORATORY 12/2/0) - 1 (2 HOURS Lecture): Characterization and identification of pathophysiological changes in blood diseases - 2 (2 HOURS Lecture): The role of the laboratory in monitoring the cardiovascular system - 3 (2 HOURS Lecture): The role of the laboratory in myocardial infarction - 4 (2 HOURS Lecture): The role of the laboratory in demyelinating diseases - 5 (2 HOURS Lecture): The role of the laboratory in myopathies - 6 (2 HOURS Lecture): The role of the laboratory in prenatal screening - 7 (2 HOURS Tutorial): Integration and links between the fundamentals of laboratory medicine, measurable parameters and their application in the possible development of new devices. KNOWLEDGE AND UNDERSTANDING: KNOW THE PROCESS OF IDENTIFYING THE BEST PATHOLOGY-RELATED PARAMETERS AND THEIR POSSIBLE IMPLEMENTATION IN MONITORING APPLIED KNOWLEDGE AND UNDERSTANDING SKILLS: Recognize the general pathophysiological picture of the disease and adopt the appropriate analytical process for diagnosis and follow-up.

Contents

Didactic Unit 1: STRUCTURE AND PROPERTIES OF THE MAIN MACROMOLECULES OF BIOLOGICAL INTEREST
(LESSON /PRACTICE / LABORATORY HOURS 10/2/0)
- 1 (2 HOURS Lesson): Introduction to biological macromolecules. Proteins as amino acid polymers; nucleic acids as nucleotide polymers; polysaccharides and lipids
-2 (2 HOURS Lecture) Elements of cellular architecture. Membranes, organelles and their functions, cell compartments, ribosomes. Cellular signaling and cellular response.
- 3 (2 HOURS Lesson): Protein structure: peptide bond, secondary and higher level protein structures. Structure-activity relationships.
- 4 (2 HOURS Lesson): Enzymes: catalysis, enzymatic kinetics (basic), role of catalysis in metabolism
- 5 (2 HOURS Lesson): Nucleic acids functionalities in protein synthesis. DNA and RNA structures. Basic concepts in transcription and translation. Chromatin structure.
- 6 (2 HOURS Practice): Use of sequences database (nucleic acids, proteins). Sequence alignment tools.
KNOWLEDGE AND UNDERSTANDING: FUNCTIONS OF MOLECULES OF BIOLOGICAL INTEREST
APPLIED KNOWLEDGE AND UNDERSTANDING: ABILITY TO CORRELATE MAJOR BIOMOLECULES STRUCTURES WITH THEIR FUNCTIONS IN PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS.
Unità didattica 2: CELLULAR METABOLISM
(LESSON /PRACTICE / LABORATORY HOURS 10/2/0)
- 1 (2 HOURS Lesson) Nutritional principles and their assimilation. Macromolecules as a source of energy.
2 (2 HOURS Lesson) Metabolism and energy; ATP, energy transfer, coupling of endo / exo-ergonic reactions.
- 3 (2 HOURS Lesson): carbohydrate metabolism. glycolysis and fermentation. glycogen metabolism
- 4 (2 HOURS Lesson): metabolism of lipids and amino acids.
- 5 (2 HOURS Lesson): cycle of tricarboxylic acids and energy production through oxidative phosphorylation. electron transport chain.
- 6 (2 HOURS Exercise): Connection of the concepts covered to the functionality of the cells; examples of pathologies related to metabolism disregulation.
KNOWLEDGE AND UNDERSTANDING: KNOWING THE MAIN BIOCHEMICAL TRANSFORMATIONS AND CONNECTING THEM TO THE MAIN CELLULAR ACTIVITIES.
APPLIED KNOWLEDGE AND UNDERSTANDING APPLIED: IDENTIFY THE MEANING OF BIOMARKER.

Learning Unit 3: FUNDAMENTALS OF LABORATORY MEDICINE.
(HOURS LECTURE/EXERCISE/LABORATORY 8/2/0)

- 1 (2 HOURS Lecture): Introduction to laboratory medicine, the analytical method, organization of the biomedical laboratory, objectives of laboratory medicine

- 2 (2 HOURS Lecture): Instrumental and biochemical data collection, differentiation of biological specimens and sources of errors

- 3 (2 HOURS Lecture): The main techniques of laboratory medicine, the concept of biomarkers and genetic predisposition to disease

-4 (2 HOURS Lecture): the concept of new devices that can be used in medicine, experimental medical devices and devices already approved by the National Institute of Health

5 (2 HOURS Exercise): The use of main scientific search engines and the define the state of the art of the disease of interest

KNOWLEDGE AND UNDERSTANDING SKILLS: KNOW THE ROLE OF LABORATORY MEDICINE IN MONITORING HEALTH AND DISEASE STATUS, UNDERSTAND MEASURABLE PARAMETERS IN BIOLOGICAL SAMPLES

APPLIED KNOWLEDGE AND CAPABILITY OF UNDERSTANDING: SELECT THE BEST MARKER OF PATHOLOGY and Hypothesize THE DEVELOPMENT OF NEW POSSIBLE TOOLS

Learning Unit 4: THE ROLE OF LABORATORY MEDICINE IN THE IDENTIFICATION AND FOLLOW-UP OF PATHOLOGIES.
(HOURS LECTURE/EXERCISE/LABORATORY 12/2/0)

- 1 (2 HOURS Lecture): Characterization and identification of pathophysiological changes in blood diseases

- 2 (2 HOURS Lecture): The role of the laboratory in monitoring the cardiovascular system

- 3 (2 HOURS Lecture): The role of the laboratory in myocardial infarction

- 4 (2 HOURS Lecture): The role of the laboratory in demyelinating diseases

- 5 (2 HOURS Lecture): The role of the laboratory in myopathies

- 6 (2 HOURS Lecture): The role of the laboratory in prenatal screening

- 7 (2 HOURS Tutorial): Integration and links between the fundamentals of laboratory medicine, measurable parameters and their application in the possible development of new devices.

KNOWLEDGE AND UNDERSTANDING: KNOW THE PROCESS OF IDENTIFYING THE BEST PATHOLOGY-RELATED PARAMETERS AND THEIR POSSIBLE IMPLEMENTATION IN MONITORING

APPLIED KNOWLEDGE AND UNDERSTANDING SKILLS: Recognize the general pathophysiological picture of the disease and adopt the appropriate analytical process for diagnosis and follow-up.

	Teaching Methods
	THE BIOCHEMISTRY ELEMENTS AND LABORATORY MEDICINE COURSE INCLUDES LECTURES AND PRACTICE FOR A TOTAL OF 48 HOURS. IN ORDER TO TAKE THE FINAL EXAM, THE STUDENT MUST HAVE ATTENDED AT LEAST 70% OF THE SCHEDULE HOURS. THE PRESENCE CHECK METHOD IS NOTIFIED BY THE TEACHER AT THE BEGINNING OF THE LESSONS.

	Verification of learning
	PROFICIENCY IS EVALUATED THROUGH ORAL EXAMINATION. THE EXAM WILL PROVIDE VERIFICATION OF: - THEORETICAL AND APPLICATED KNOWLEDGE, - LANGUAGE SKILLS. THE EVALUATION CAN BE CUM LAUDE FOR STUDENTS SHOWING AN OUTSTANDING KNOWLEDGE OF THE TOPICS OF THE COURSE.

	Texts
	SAMAJA ET AL. CHIMICA E BIOCHIMICA PER LE LAUREE TRIENNALI DELL’AREA BIOMEDICA. ED. PICCIN BIOCHIMICA CLINICA E MEDICINA DI LABORATORIO AUTORE: M. CIACCIO, G. LIPPI EDITORE: EDISES MEDICINA DI LABORATORIO - LOGICA E PATOLOGIA CLINICA AUTORE: ANTONOZZI - GULLETTA, EDITORE: PICCIN MATERIALE DIDATTICO FORNITO DAL DOCENTE SUPPLEMENTARY TEACHING MATERIAL WILL BE AVAILABLE ON THE UNIVERSITY E-LEARNING PLATFORM (HTTP://ELEARNING.UNISA.IT) ACCESSIBLE TO STUDENTS USING THEIR OWN UNIVERSITY CREDENTIALS.

Texts

SAMAJA ET AL. CHIMICA E BIOCHIMICA PER LE LAUREE TRIENNALI
DELL’AREA BIOMEDICA. ED. PICCIN

BIOCHIMICA CLINICA E MEDICINA DI LABORATORIO
AUTORE: M. CIACCIO, G. LIPPI
EDITORE: EDISES

MEDICINA DI LABORATORIO - LOGICA E PATOLOGIA CLINICA
AUTORE: ANTONOZZI - GULLETTA,
EDITORE: PICCIN

MATERIALE DIDATTICO FORNITO DAL DOCENTE

SUPPLEMENTARY TEACHING MATERIAL WILL BE AVAILABLE ON THE UNIVERSITY E-LEARNING PLATFORM (HTTP://ELEARNING.UNISA.IT) ACCESSIBLE TO STUDENTS USING THEIR OWN UNIVERSITY CREDENTIALS.

	More Information
	THE COURSE IS HELD IN ITALIAN

Lessons Timetable

BETA VERSION Data source ESSE3 [Ultima Sincronizzazione: 2025-03-26]

ALBINO CARRIZZO | ELEMENTS OF BIOCHEMISTRY AND LABORATORY MEDICINE