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APPLIED BIOCHEMISTRY

Maria PASCALE APPLIED BIOCHEMISTRY

0760100018
DIPARTIMENTO DI FARMACIA
CORSO DI LAUREA MAGISTRALE A CICLO UNICO DI 5 ANNI
PHARMACY
2015/2016

OBBLIGATORIO
YEAR OF COURSE 3
YEAR OF DIDACTIC SYSTEM 2010
PRIMO SEMESTRE
CFUHOURSACTIVITY
540LESSONS
MARIA PASCALE T Curriculum
Objectives
THE PROGRAMS ARE DESIGNED TO PROVIDE STUDENTS WITH A THOROUGH GROUNDING THE THEORETICAL PRINCIPLES UNDERLYING THE MOST IMPORTANT METHODOLOGIES USED IN THE BIOCHEMICAL STUDIES.

IN AGREEMENT WITH THE DUBLIN DESCRIPTORS, AT THE END OF THE COURSE THE STUDENT WILL DEMONSTRATE:
1. A BASIC KNOWLEDGE AND UNDERSTANDING OF THE THEORETICAL FOUNDATIONS NECESSARY TO IDENTIFY AND CHARACTERIZE THE FUNCTIONAL GROUPS AND/OR BIOLOGICAL PROPERTIES OF POTENTIAL BIOLOGICAL AND PHARMACEUTICAL INTEREST. IN PARTICULAR, THE LOGIC OF EXPERIMENTAL DESIGN AND DATA ANALYSIS IS EMPHASIZED;
2. THE ABILITY TO ANALYZE, DISCUSS AND RUN REPORTS ON SIMPLE LABORATORY EXPERIMENTS;
3. AN INDEPENDENT AND CRITICAL ASSESSEMENT OF ANY DIFFERENT EXPERIMENTAL PROCEDURE PRESENT IN THE COURSE TOPICS;
4. THE ABILITY TO PROPERLY EXPOSE THE KNOWLEDGE ACQUIRED USING THEAPPROPRIATE SCIENTIFIC LANGUAGE;
5. THE ABILITY TO COMMUNICATE IN A CRITICAL MANNER THE TECHNIQUES AND THE ARGUMENTS STUDIED AND TO DESCRIBE THE ARGUMENTS WITH A INTER-DISCIPLINARY APPROACH.
Prerequisites
BASIC KNOWLEDGE ACHIEVEMENT IN GENERAL AND INORGANIC CHEMISTRY, ORGANIC CHEMISTRY, PHYSICS, BIOLOGY AND BIOCHEMISTRY
Contents
EXPERIMENTAL SYSTEMS IN BIOCHEMICAL STUDIES: IN VIVO AND IN VITRO MODELS.
UV-VIS SPECTROSCOPY: FIRST ELEMENTS, APPLICATIONS AND INSTRUMENTATION, ASSAY METHODS FOR PROTEIN MEASURING.
SEDIMENTATION AND CENTRIFUGATION: PHYSICAL LAWS, PREPARATIVE AND ANALYTICAL APPLICATIONS.
ELECTROPHORESIS: FIRST ELEMENTS AND PHYSICAL LAWS, ELECTROPHORESIS IN SEMISOLID MEDIA: AGAROSE GELS AND POLYACRILAMIDE GELS.
SDS-PAGE AND MOLECULAR MASS DETERMINATION OF PROTEINS, NATIVE PAGE, ISOELECTROFOCUSING AND PROTEIN PI.
WESTERN AND SOUTH-WESTERN BLOTTING.
AGAROSE AND POLYACRILAMIDE GEL ELECTROPHORESIS OF NUCLEIC ACIDS.
CHROMATOGRAPHY: FIRST ELEMENTS AND THEORETICAL PRINCIPLES, AFFINITY CHROMATOGRAPHY.
RADIOCHEMISTRY: NUCLEAR REACTIONS, RADIOISOTOPES, RADIOACTIVE DECAY.
MATTER-RADIATION INTERACTIONS: SAFETY ELEMENTS IN RADIOPROTECTION.
DETECTION AND MEASURING OF RADIOACTIVITY: AUTORADIOGRAPHY, GAS IONIZATION METHODS, SCINTILLATION METHODS, USES OF RADIOISOTOPIC TRACERS IN BIOCHEMICAL, BIOMOLECULAR AND BIOMEDICAL ANALYSES.
IMMUNOCHEMISTRY: MONOCLONAL (MAB) AND POLYCLONAL (PAB) ANTIBODIES PRODUCTION.
IN VIVO AND IN VITRO RADIOLABELING OF PROTEINS AND NUCLEIC ACIDS.
IMMUNOPRECIPITATION, ANTIBODY LABELING. IMMUNOASSAYS: RIA, IRMA, ELISA.
NUCLEIC ACIDS ANALYSIS:NORTHERN AND SOUTHERN ANALYSES, RESTRICTION ENZYMES, MODIFICATION ENZYMES AND THEIR APPLICATIONS.
POLYMERASE CHAIN REACTION (PCR). SENSITIVITY AND GENERAL APPLICATIONS OF PCR.
Teaching Methods
THE COURSE INCLUDES LECTURES AND TUTORIALS.
Verification of learning
THE VERIFICATION OF HAVING ACHIEVED, BY THE STUDENTS, THE OBJECTIVES IS DONE BY A FINAL WRITTEN TEST AND A FOLLOWING ORAL EXAMINATION.
Texts

WILSON, KEITH & WALKER, JOHN -PRINCIPLES AND TECHNIQUES OF BIOCHEMISTRY AND MOLECULAR BIOLOGY - 6TH ED. CAMBRIDGE UNIVERSITY PRESS, 2005

(ITALIAN EDITION: WILSON KEITH & WALKER, JOHN - BIOCHIMICA E BIOLOGIA MOLECOLARE: PRINCIPI E TECNICHE - RAFFAELLO CORTINA ED. (ISBN 88-6030-066-5)
More Information
THE COURSE IS TAUGHT IN ITALIAN

THE ATTENDANCE IS MANDATORY

DEPARTMENT OF PHARMACY
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