Agostino CASAPULLO | PHYSICAL METHODS IN ORGANIC CHEMISTRY-APPLIED BIOCHEMISTRY

cod. 0760200015

PHYSICAL METHODS IN ORGANIC CHEMISTRY-APPLIED BIOCHEMISTRY

	0760200015
	DIPARTIMENTO DI FARMACIA
	EQF7
	PHARMACEUTICAL CHEMISTRY AND TECHNOLOGY
	2017/2018

PERCORSO COMUNE Cohort 2015/2016

	OBBLIGATORIO
	YEAR OF COURSE 3
	YEAR OF DIDACTIC SYSTEM 2010
	PRIMO SEMESTRE

TEACHING UNITS

	SSD	CFU	HOURS	ACTIVITY	TYPE OF ACTIVITY
1	METODI FISICI IN CHIMICA ORGANICA MODULO DI METODI FISICI IN CHIMICA ORGANICA-BIOCHIMICA APPLICATA
	CHIM/06	5	40	LESSONS	BASIC COMPULSORY SUBJECTS
2	BIOCHIMICA APPLICATA MODULO DI METODI FISICI IN CHIMICA ORGANICA-BIOCHIMICA APPLICATA
	BIO/10	5	40	LESSONS	COMPULSORY SUBJECTS, CHARACTERISTIC OF THE CLASS

PROFESSORS

	MARIA PASCALE2 T Curriculum
	AGOSTINO CASAPULLO1 Curriculum

	Objectives
	Teaching is aimed at acquiring knowledge and understanding of the structure-function relationship of biological macromolecules, biochemical and molecular mechanisms of cellular metabolism (anabolic and catabolic) and its regulation; metabolic relationships between the various tissues and organs, and the main streets of signal transduction mediated by hormones. The student applying the acquired skills, will be able to outline the overall picture of the metabolism at the molecular and cellular level, and apply it to the study of relations between different metabolic pathways

Teaching is aimed at acquiring knowledge and understanding of the structure-function relationship of biological macromolecules, biochemical and molecular mechanisms of cellular metabolism (anabolic and catabolic) and its regulation; metabolic relationships between the various tissues and organs, and the main streets of signal transduction mediated by hormones.
The student applying the acquired skills, will be able to outline the overall picture of the metabolism at the molecular and cellular level, and apply it to the study of relations between different metabolic pathways

	Prerequisites
	TEACHING UNIT 1: PHYSICAL METHODS IN ORGANIC CHEMISTRY BASIC KNOWLEDGE ACHIEVEMENT IN GENERAL AND INORGANIC CHEMISTRY, ORGANIC CHEMISTRY, PHYSICS AND PHYSICAL CHEMISTRY TEACHING UNIT 2: APPLIED BIOCHEMISTRY ACHIEVEMENT OF BASIC KNOWLEDGES IN BIOLOGY, INORGANIC CHEMISTRY, ORGANIC CHEMISTRY, BIOCHEMISTRY.

	Contents
	THIS CLASS IS COMPOSED OF TWO MODULES: MODULE 1 (PHYSICAL METHODS IN ORGANIC CHEMISTRY) AND MODULE 2 (APPLIED BIOCHEMISTRY), 40 HOURS EACH ONE (5 CFU) OF LECTURES. TEACHING UNIT 1: PHYSICAL METHODS IN ORGANIC CHEMISTRY 1.ELECTROMAGNETIC RADIATION: GENERAL CHARACTERISTICS, QUANTUM MOLECULAR PROPERTIES, 2.INTRODUCTION TO MOLECULAR SPECTROSCOPY. 3.INFRARED SPECTROSCOPY (IR): MODEL DESCRIPTION OF THE VIBRATIONAL PROPERTIES: THE CLASSICAL AND QUANTUM HARMONIC OSCILLATOR. THE USE OF IR FOR CHEMICAL DIAGNOSTICS. APPLICATIONS. 4.ULTRAVIOLET-VISIBLE SPECTROSCOPY (UV-VIS): MOLECULAR ORBITALS AND LCAO THEORY. ELECTRONIC TRANSITIONS. BEER-LAMBERT LAW. QUANTITATIVE ASPECTS OF UV SPECTROSCOPY IN RELATION TO QUALITY CONTROL. PREDICTION OF LAMBDA-MAX THROUGH WOODWARD RULES. APPLICATIONS. 5.CIRCULAR DICHROISM (CD) SPECTROSCOPY: PRINCIPLES OF CIRCULAR DICHROISM (CD) AND POLARIMETRY. OPERATION AND USE OF A CD SPECTROPHOTOMETER. USING CD SPECTROSCOPY FOR THE DETERMINATION OF THE CONFORMATION AND CONFIGURATION OF ORGANIC MOLECULES. 6.SURFACE PLASMON RESONANCE (SPR): GENERAL PRINCIPLES, INSTRUMENTATION AND SCOPE. 7.MASS SPECTROMETRY ION SOURCES AND ANALYZERS. NOTES ON THE PHYSICAL THEORY CONCERNING THE PRODUCTION AND THE OBSERVATION OF MOLECULAR IONS. IONIZATION TECHNIQUES. ANALYZERS. TANDEM MASS SPECTROMETRY AND APPLICATIONS. 8.NUCLEAR MAGNETIC RESONANCE (NMR): GENERAL CHARACTERISTICS AND THEORY OF CHEMICAL SHIFTS AND COUPLING OF SPIN. 1H-NMR AND 13C-NMR. TWO-DIMENSIONAL NMR SPECTROSCOPY. TEACHING UNIT 2: APPLIED BIOCHEMISTRY 1.EXPERIMENTAL MODELS IN VIVO AND IN VITRO. CELL AND MICROBIAL CULTURES. CELL COUNT TECHNIQUES. FACS ANALYSIS AND ITS APPLICATIONS. 2.UV-VIS SPECTROPHOTOMETRY. SPECTROFLUORIMETRY. 3.SEDIMENTATION AND CENTRIFUGATION. PREPARATION AND FRACTIONATION OF CELL AND TISSUE HOMOGENATES. 4.PROTEIN ASSAYS. AMINO ACID ANALYSIS. PROTEIN SEQUENCE. PROTEOLYSIS AND PEPTIDES: MASS SPECTROMETRY IN THE PROTEOME ANALYSIS. 5.ELECTROPHORESIS: AGAROSE AND POLYACRYLAMIDE GELS. ELECTROPHORESIS IN LIQUID AND IN GELS. SDS-PAGE AND NATIVE ELECTROPHORERSIS. GRADIENT GELS. SDS-PAGE. NATIVE PAGE. ISOELECTROFOCUSING. DETECTION, QUANTITATION AND RECOVERY OF PROTEIN FROM GELS. 6.WESTERN AND SOUTH-WESTERN BLOTTING. NUCLEIC ACIDS ELECTROPHORESIS. PFGE. SOUTHERN AND NORTHERN BLOTTING. 7.DNA SEQUENCING: SANGER AND MAXAM-GILBERT METHODS. 8.CHROMATOGRAPHY: GENERAL PRINCIPLES. TLC. COLUMN CHROMATOGRAPHY: LPLC, HPLC, FPLC. ADSORPTION (HIC), PARTITION (NPC , RPC, IP-RPC) CHROMATOGRAPHY, IEC, GFC, AC. WORKING OUT OF PROTEIN PURIFICATION PROTOCOLS. 9.RADIOCHEMISTRY AND RADIOISOTOPES. DETECTION AND MEASURING OF RADIOACTIVITY. IMMUNOCHEMISTRY: MAB AND PAB ANTIBODIES PRODUCTION. RIA, IRMA, ELISA. 10.RESTRICTION AND MODIFICATION ENZYMES, POLYMERASE CHAIN REACTION (PCR): SENSITIVITY AND GENERAL APPLICATIONS. 11.CLONING VECTORS AND MOLECULAR CLONING. PRODUCTION OF RECOMBINANT PROTEINS.

Contents

THIS CLASS IS COMPOSED OF TWO MODULES: MODULE 1 (PHYSICAL METHODS IN ORGANIC CHEMISTRY) AND MODULE 2 (APPLIED BIOCHEMISTRY), 40 HOURS EACH ONE (5 CFU) OF LECTURES.
TEACHING UNIT 1: PHYSICAL METHODS IN ORGANIC CHEMISTRY
1.ELECTROMAGNETIC RADIATION: GENERAL CHARACTERISTICS, QUANTUM MOLECULAR PROPERTIES,
2.INTRODUCTION TO MOLECULAR SPECTROSCOPY.
3.INFRARED SPECTROSCOPY (IR): MODEL DESCRIPTION OF THE VIBRATIONAL PROPERTIES: THE CLASSICAL AND QUANTUM HARMONIC OSCILLATOR. THE USE OF IR FOR CHEMICAL DIAGNOSTICS. APPLICATIONS.
4.ULTRAVIOLET-VISIBLE SPECTROSCOPY (UV-VIS): MOLECULAR ORBITALS AND LCAO THEORY. ELECTRONIC TRANSITIONS. BEER-LAMBERT LAW. QUANTITATIVE ASPECTS OF UV SPECTROSCOPY IN RELATION TO QUALITY CONTROL. PREDICTION OF LAMBDA-MAX THROUGH WOODWARD RULES. APPLICATIONS.
5.CIRCULAR DICHROISM (CD) SPECTROSCOPY: PRINCIPLES OF CIRCULAR DICHROISM (CD) AND POLARIMETRY. OPERATION AND USE OF A CD SPECTROPHOTOMETER. USING CD SPECTROSCOPY FOR THE DETERMINATION OF THE CONFORMATION AND CONFIGURATION OF ORGANIC MOLECULES.
6.SURFACE PLASMON RESONANCE (SPR): GENERAL PRINCIPLES, INSTRUMENTATION AND SCOPE.
7.MASS SPECTROMETRY ION SOURCES AND ANALYZERS. NOTES ON THE PHYSICAL THEORY CONCERNING THE PRODUCTION AND THE OBSERVATION OF MOLECULAR IONS. IONIZATION TECHNIQUES. ANALYZERS. TANDEM MASS SPECTROMETRY AND APPLICATIONS.
8.NUCLEAR MAGNETIC RESONANCE (NMR): GENERAL CHARACTERISTICS AND THEORY OF CHEMICAL SHIFTS AND COUPLING OF SPIN. 1H-NMR AND 13C-NMR. TWO-DIMENSIONAL NMR SPECTROSCOPY.

TEACHING UNIT 2: APPLIED BIOCHEMISTRY
1.EXPERIMENTAL MODELS IN VIVO AND IN VITRO. CELL AND MICROBIAL CULTURES. CELL COUNT TECHNIQUES. FACS ANALYSIS AND ITS APPLICATIONS.
2.UV-VIS SPECTROPHOTOMETRY. SPECTROFLUORIMETRY. 3.SEDIMENTATION AND CENTRIFUGATION. PREPARATION AND FRACTIONATION OF CELL AND TISSUE HOMOGENATES.
4.PROTEIN ASSAYS. AMINO ACID ANALYSIS. PROTEIN SEQUENCE. PROTEOLYSIS AND PEPTIDES: MASS SPECTROMETRY IN THE PROTEOME ANALYSIS.
5.ELECTROPHORESIS: AGAROSE AND POLYACRYLAMIDE GELS. ELECTROPHORESIS IN LIQUID AND IN GELS. SDS-PAGE AND NATIVE ELECTROPHORERSIS. GRADIENT GELS. SDS-PAGE. NATIVE PAGE. ISOELECTROFOCUSING. DETECTION, QUANTITATION AND RECOVERY OF PROTEIN FROM GELS.
6.WESTERN AND SOUTH-WESTERN BLOTTING. NUCLEIC ACIDS ELECTROPHORESIS. PFGE. SOUTHERN AND NORTHERN BLOTTING. 7.DNA SEQUENCING: SANGER AND MAXAM-GILBERT METHODS. 8.CHROMATOGRAPHY: GENERAL PRINCIPLES. TLC. COLUMN CHROMATOGRAPHY: LPLC, HPLC, FPLC. ADSORPTION (HIC), PARTITION (NPC , RPC, IP-RPC) CHROMATOGRAPHY, IEC, GFC, AC. WORKING OUT OF PROTEIN PURIFICATION PROTOCOLS. 9.RADIOCHEMISTRY AND RADIOISOTOPES. DETECTION AND MEASURING OF RADIOACTIVITY. IMMUNOCHEMISTRY: MAB AND PAB ANTIBODIES PRODUCTION. RIA, IRMA, ELISA. 10.RESTRICTION AND MODIFICATION ENZYMES, POLYMERASE CHAIN REACTION (PCR): SENSITIVITY AND GENERAL APPLICATIONS.
11.CLONING VECTORS AND MOLECULAR CLONING. PRODUCTION OF RECOMBINANT PROTEINS.

	Teaching Methods
	THIS CLASS IS COMPOSED OF TWO MODULES AND CONSISTS OF 80 HOURS OF LECTURES SUPPORTED BY SLIDE PRESENTATION. CLASS ATTENDANCE IS COMPULSORY. STUDENTS ARE ALLOWED TO GET THE EXAMINATION IF THEIR ATTENDANCE IS GREATER THAN 70%. CLASS ATTENDANCE IS CHECKED BY BADGE ELECTRONIC DETECTION

	Verification of learning
	THE EXAM FOR EACH MODULE IS PASSED WITH GRADES ON A SCALE OF 30. THE FINAL GRADE IS CALCULATED BY THE MEAN OF THE GRADES GAINED IN EACH MODULE. THE EXAM INCLUDES A WRITING AND AN ORAL TEST. THE FIRST STEP IS THE WRITING TEST, PREPARATORY FOR THE ORAL EXAM, AND CONSISTS OF ESSAY QUESTIONS ON THE TOPICS STUDIED. IT LASTS ABOUT 40-60 MINUTES, AND IS DUE TO UNDERSTAND THE STUDENT COMPREHENSION OF THE TOPICS AND HIS ABILITY IN THE APPLICATION OF THEORETICAL KNOWLEDGE. THE ORAL TEST CONSISTS IN QUESTIONS ON THE TOPICS STUDIED, TO EVALUATE THE STUDENTS LEVEL OF KNOWLEDGE AND COMPREHENSION, AND THEIR PRESENTATION SKILLS USING A CORRECT SCIENTIFIC LANGUAGE. THE FINAL GRADE IS THE MEAN OF THE GRADES GAINED IN EACH TEST

Verification of learning

THE EXAM FOR EACH MODULE IS PASSED WITH GRADES ON A SCALE OF 30. THE FINAL GRADE IS CALCULATED BY THE MEAN OF THE GRADES GAINED IN EACH MODULE.
THE EXAM INCLUDES A WRITING AND AN ORAL TEST. THE FIRST STEP IS THE WRITING TEST, PREPARATORY FOR THE ORAL EXAM, AND CONSISTS OF ESSAY QUESTIONS ON THE TOPICS STUDIED. IT LASTS ABOUT 40-60 MINUTES, AND IS DUE TO UNDERSTAND THE STUDENT COMPREHENSION OF THE TOPICS AND HIS ABILITY IN THE APPLICATION OF THEORETICAL KNOWLEDGE. THE ORAL TEST CONSISTS IN QUESTIONS ON THE TOPICS STUDIED, TO EVALUATE THE STUDENTS LEVEL OF KNOWLEDGE AND COMPREHENSION, AND THEIR PRESENTATION SKILLS USING A CORRECT SCIENTIFIC LANGUAGE. THE FINAL GRADE IS THE MEAN OF THE GRADES GAINED IN EACH TEST

	Texts
	TEACHING UNIT 1: PHYSICAL METHODS IN ORGANIC CHEMISTRY 1. HESSE, MEIER, ZEEH, METODI SPETTROSCOPICI NELLA CHIMICA ORGANICA, EDISES, NAPOLI 2. R.M. SILVERSTEIN, F.X. WEBSTER, IDENTIFICAZIONE SPETTROSCOPICA DI COMPOSTI ORGANICI, AMBROSIANA EDITRICE 3. CHIAPPE, D'ANDREA, TECNICHE SPETTROSCOPICHE E IDENTIFICAZIONE DI COMPOSTI ORGANICI, EDIZIONI ETS 4. HOLLER, SKOOG, GROUCH, CHIMICA ANALITICA STRUMENTALE, EDISES 5. PEDULLI, METODI FISICI IN CHIMICA ORGANICA, PICCIN ED. TEACHING UNIT 2: APPLIED BIOCHEMISTRY WILSON, KEITH & WALKER, JOHN - PRINCIPLES AND TECHNIQUES OF BIOCHEMISTRY AND MOLECULAR BIOLOGY - CAMBRIDGE UNIVERSITY PRESS (ISBN-13 978-0-521-53581-6

Texts

TEACHING UNIT 1: PHYSICAL METHODS IN ORGANIC CHEMISTRY
1. HESSE, MEIER, ZEEH, METODI SPETTROSCOPICI NELLA CHIMICA ORGANICA, EDISES, NAPOLI
2. R.M. SILVERSTEIN, F.X. WEBSTER, IDENTIFICAZIONE SPETTROSCOPICA DI COMPOSTI ORGANICI, AMBROSIANA EDITRICE
3. CHIAPPE, D'ANDREA, TECNICHE SPETTROSCOPICHE E IDENTIFICAZIONE DI COMPOSTI ORGANICI, EDIZIONI ETS
4. HOLLER, SKOOG, GROUCH, CHIMICA ANALITICA STRUMENTALE, EDISES
5. PEDULLI, METODI FISICI IN CHIMICA ORGANICA, PICCIN ED.

TEACHING UNIT 2: APPLIED BIOCHEMISTRY
WILSON, KEITH & WALKER, JOHN - PRINCIPLES AND TECHNIQUES OF BIOCHEMISTRY AND MOLECULAR BIOLOGY - CAMBRIDGE UNIVERSITY PRESS (ISBN-13 978-0-521-53581-6

	More Information
	THE COURSE IS TAUGHT IN ITALIAN THE ATTENDANCE IS MANDATORY LOCATION: DEPARTMENT OF PHARMACY

BETA VERSION Data source ESSE3 [Ultima Sincronizzazione: 2019-05-14]

Agostino CASAPULLO | PHYSICAL METHODS IN ORGANIC CHEMISTRY-APPLIED BIOCHEMISTRY