STEFANIA MARTUCCIELLO | ANALYTICAL AND BIOMOLECULAR METHODS

cod. 0512800031

ANALYTICAL AND BIOMOLECULAR METHODS

	0512800031
	DEPARTMENT OF CHEMISTRY AND BIOLOGY "ADOLFO ZAMBELLI"
	EQF6
	BIOLOGICAL SCIENCES
	2019/2020

PERCORSO COMUNE

	OBBLIGATORIO
	YEAR OF COURSE 3
	YEAR OF DIDACTIC SYSTEM 2016
	ANNUALE

TEACHING UNITS

	SSD	CFU	HOURS	ACTIVITY	TYPE OF ACTIVITY
1	METODOLOGIE BIOMOLECOLARI (MODULO DI METODOLOGIE ANALITICHE E BIOMOLECOLARI)
	BIO/10	5	40	LESSONS	COMPULSORY SUBJECTS, CHARACTERISTIC OF THE CLASS
	BIO/10	1	12	LAB	COMPULSORY SUBJECTS, CHARACTERISTIC OF THE CLASS
2	METODOLOGIE ANALITICHE (MODULO DI METODOLOGIE ANALITICHE E BIOMOLECOLARI)
	CHIM/01	4	32	LESSONS	SUPPLEMENTARY COMPULSORY SUBJECTS
	CHIM/01	2	24	LAB	SUPPLEMENTARY COMPULSORY SUBJECTS

PROFESSORS

	IVANA CAPUTO1 T Curriculum
	STEFANIA MARTUCCIELLO1 Curriculum
	MARIA STRIANESE2 Curriculum

	Objectives
	Knowledge and Understanding THE AIM OF THE COURSE IS TO GIVE KNOWLEDGE OF THE BASICS OF THE MAJOR CLASSICAL METHODS OF ANALYSIS AND OF THE MAIN METHODS OF INVESTIGATION IN BIOCHEMISTRY AND MOLECULAR BIOLOGY. Application of knowledge and understanding THE COURSE ALSO AIMS TO STIMULATE THE DEVELOPMENT OF THE ABILITY TO INTERPRET AND PERFORM AN ANALYTICAL PROTOCOL, AND DESCRIBE THE RESULTS WITH MATHEMATICAL RIGOR, AS WELL AS TO STIMULATE THE DEVELOPMENT OF THE ABILITY TO UNDERSTAND AND APPLY AN EXPERIMENTAL PROTOCOL CONCERNING THE HANDLING OF BIOMOLECULES.

Knowledge and Understanding
THE AIM OF THE COURSE IS TO GIVE KNOWLEDGE OF THE BASICS OF THE MAJOR CLASSICAL METHODS OF ANALYSIS AND OF THE MAIN METHODS OF INVESTIGATION IN BIOCHEMISTRY AND MOLECULAR BIOLOGY.

Application of knowledge and understanding
THE COURSE ALSO AIMS TO STIMULATE THE DEVELOPMENT OF THE ABILITY TO INTERPRET AND PERFORM AN ANALYTICAL PROTOCOL, AND DESCRIBE THE RESULTS WITH MATHEMATICAL RIGOR, AS WELL AS TO STIMULATE THE DEVELOPMENT OF THE ABILITY TO UNDERSTAND AND APPLY AN EXPERIMENTAL PROTOCOL CONCERNING THE HANDLING OF BIOMOLECULES.

	Prerequisites
	ORGANIC CHEMISTRY IS REQUIRED. SKILLS IN MATHEMATICS, PHYSICS, BIOCHEMISTRY AND MOLECULAR BIOLOGY ARE USEFUL.

	Contents
	THE COURSE IS WELL-STRUCTURED INTO TWO MODULES, EACH OF 6 CFU, NAMED MODULE OF ANALYTICAL CHEMISTRY AND MODULE OF BIOMOLECULAR METHODS. EACH MODULE INCLUDES THEORETICAL AND PRACTICAL LECTURES. SUBJECTS OF THE MODULE IF ANALYTICAL CHEMISTRY ARE: STOICHIOMETRY AND BASIC OPERATION OF ANALYTICAL CHEMISTRY. MAJOR PHASES OF CHEMICAL ANALYSIS. ERRORS IN ANALYTICAL DETERMINATIONS. SAMPLING TECHNIQUES AND PRETREATMENT. THE ACID-BASE, SOLUBILITY AND COMPLEXATION EQUILIBRIA IN CHEMICAL ANALYSIS. APPLICATIONS OF ACID-BASE AND COMPLEXATION REACTIONS IN THE FRAME OF QUANTITATIVE AND QUALITATIVE ANALYSIS. THE REDOX BALANCE CHEMICAL ANALYSIS. APPLICATION OF REDOX REACTIONS IN THE FRAME OF QUANTITATIVE AND QUALITATIVE ANALYSIS. UV AND IR SPECTROPHOTOMETRY. ELECTROCHEMICAL METHODS. CHROMATOGRAPHY. SUBJECTS OF THE MODULE OB BIOMOLECULAR METHODS ARE: CELL CULTURES AND MICROSCOPY. METHODS OF HOMOGENIZATION OF CELLS AND TISSUES. CENTRIFUGATION. PROTEINS AND NUCLEIC ACIDS PRECIPITATION. CHROMATOGRAPHY AND SPECTROPHOTOMETRY APPLIED TO BIOLOGICAL MACROMOLECULES. ELECTROPHORESIS OF PROTEINS AND NUCLEIC ACIDS. HYBRIDIZATION AND BLOTTING METHODS. IMMUNOLOGICAL AND IMMUNOENZYMATIC METHODS. ELEMENTS OF MASS SPECTROMETRY. CLONING METHODS AND PCR. INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY.

Contents

THE COURSE IS WELL-STRUCTURED INTO TWO MODULES, EACH OF 6 CFU, NAMED MODULE OF ANALYTICAL CHEMISTRY AND MODULE OF BIOMOLECULAR METHODS. EACH MODULE INCLUDES THEORETICAL AND PRACTICAL LECTURES.

SUBJECTS OF THE MODULE IF ANALYTICAL CHEMISTRY ARE:
STOICHIOMETRY AND BASIC OPERATION OF ANALYTICAL CHEMISTRY. MAJOR PHASES OF CHEMICAL ANALYSIS. ERRORS IN ANALYTICAL DETERMINATIONS. SAMPLING TECHNIQUES AND PRETREATMENT. THE ACID-BASE, SOLUBILITY AND COMPLEXATION EQUILIBRIA IN CHEMICAL ANALYSIS. APPLICATIONS OF ACID-BASE AND COMPLEXATION REACTIONS IN THE FRAME OF QUANTITATIVE AND QUALITATIVE ANALYSIS. THE REDOX BALANCE CHEMICAL ANALYSIS. APPLICATION OF REDOX REACTIONS IN THE FRAME OF QUANTITATIVE AND QUALITATIVE ANALYSIS. UV AND IR SPECTROPHOTOMETRY. ELECTROCHEMICAL METHODS. CHROMATOGRAPHY.

SUBJECTS OF THE MODULE OB BIOMOLECULAR METHODS ARE:
CELL CULTURES AND MICROSCOPY. METHODS OF HOMOGENIZATION OF CELLS AND TISSUES. CENTRIFUGATION. PROTEINS AND NUCLEIC ACIDS PRECIPITATION. CHROMATOGRAPHY AND SPECTROPHOTOMETRY APPLIED TO BIOLOGICAL MACROMOLECULES. ELECTROPHORESIS OF PROTEINS AND NUCLEIC ACIDS. HYBRIDIZATION AND BLOTTING METHODS. IMMUNOLOGICAL AND IMMUNOENZYMATIC METHODS. ELEMENTS OF MASS SPECTROMETRY. CLONING METHODS AND PCR. INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY.

	Teaching Methods
	LECTURES IN THE CLASSROOM WITH PROJECTION OF TEACHING MATERIALS IN ELECTRONIC FORM. EXERCISES IN CLASS. LABORATORY PRACTISES. 108 HOURS OF LESSONS, EXERCISES AND LABORATORY ARE SPLIT IN TWO MODULES AS FOLLOWS: I MODULE(BIOMOLECULAR METHODS) LESSONS (5 CFU, 40 HOURS). LABORATORY (1 CFU, 12 HOURS). II MODULE (ANALYTICAL CHEMISTRY) LESSONS (4 CFU, 32 HOURS). LABORATORY (2 CFU, 24 HOURS).

	Verification of learning
	THE ACHIEVEMENT OF TEACHING AIMS WILL BE VERIFIED BY MEANS OF A WRITTEN EXAMINATION CONSISISTING OF TWO TEXTS FOR THE TWO MODULES, EACH WITH A DURATION OF 1 HOUR. EACH TEST CONSISTS OF A COMBINATION OF EXERCICES AND QUESTIONS WITH MULTIPLE, DIRECT OR OPEN ANSWERS. THE EXAMINATION IS ON THE WHOLE AIMED TO VERIFY THE ACQUISITION OF: KNOWDLEDGE AND EXPERTISES ON METHODS TO STUDY MOLECULES AND MACROMOLECULES; ABILITY TO CORRECTLY APPLY THEORETICAL KNOWLEDGE; ABILITY TO UNDERSTAND PROPOSED PROBLEMS; ADEQUATE LANGUAGE SKILLS.RIGHTS ANSWERS TO QUESTIONS ON BASICAL TOPICS WILL BE CONSIDERED NECESSARY TO PASS THE EXAM. THE FINALE VOTE WILL BE THE MEAN OF VOTES OBTAINED FROM EACH TEST.

Verification of learning

THE ACHIEVEMENT OF TEACHING AIMS WILL BE VERIFIED BY MEANS OF A WRITTEN EXAMINATION CONSISISTING OF TWO TEXTS FOR THE TWO MODULES, EACH WITH A DURATION OF 1 HOUR. EACH TEST CONSISTS OF A COMBINATION OF EXERCICES AND QUESTIONS WITH MULTIPLE, DIRECT OR OPEN ANSWERS.
THE EXAMINATION IS ON THE WHOLE AIMED TO VERIFY THE ACQUISITION OF: KNOWDLEDGE AND EXPERTISES ON METHODS TO STUDY MOLECULES AND MACROMOLECULES; ABILITY TO CORRECTLY APPLY THEORETICAL KNOWLEDGE; ABILITY TO UNDERSTAND PROPOSED PROBLEMS; ADEQUATE LANGUAGE SKILLS.RIGHTS ANSWERS TO QUESTIONS ON BASICAL TOPICS WILL BE CONSIDERED NECESSARY TO PASS THE EXAM.
THE FINALE VOTE WILL BE THE MEAN OF VOTES OBTAINED FROM EACH TEST.

	Texts
	FOR THE MODULE OF BIOMOLECULAR METHODS THE FOLLOWING TEXT IS ADVISED: STOPPINI-BELLOTTI. BIOCHIMICA APPLICATA-EDISES ALTERNATIVE TEXTS ARE : WILSON, WALKER. BIOCHIMICA E BIOLOGIA MOLECOLARE PRINCIPI E TECNICHE-RAFFAELLO CORTINA EDITORE; REED, HOLMES, WEYERS E JONES, METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI, ZANICHELLI.

	More Information
	PARTICIPATION IN THE COURSE IS STRONGLY RECOMMENDED.

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STEFANIA MARTUCCIELLO | ANALYTICAL AND BIOMOLECULAR METHODS