Carmine VECCHIONE | C.I. BIOCHIMICA E BIOLOGIA

cod. 1060200011

C.I. BIOCHIMICA E BIOLOGIA

	1060200011
	DIPARTIMENTO DI MEDICINA, CHIRURGIA E ODONTOIATRIA "SCUOLA MEDICA SALERNITANA"
	EQF7
	DENTISTRY
	2016/2017

PERCORSO COMUNE

	OBBLIGATORIO
	YEAR OF COURSE 2
	YEAR OF DIDACTIC SYSTEM 2015
	PRIMO SEMESTRE

TEACHING UNITS

	SSD	CFU	HOURS	ACTIVITY	TYPE OF ACTIVITY
1	C.I. BIOCHIMICA E BIOLOGIA - MOD. BIOCHIMICA
	BIO/10	2	22	LESSONS	BASIC COMPULSORY SUBJECTS
2	C.I. BIOCHIMICA E BIOLOGIA - MOD. BIOLOGIA MOLECOLARE
	BIO/11	7	77	LESSONS	BASIC COMPULSORY SUBJECTS
3	C.I. BIOCHIMICA E BIOLOGIA - MOD. SCIENZE E TECNICHE DI MEDICINA DI LABORATORIO
	MED/46	4	44	LESSONS	SUPPLEMENTARY COMPULSORY SUBJECTS

PROFESSORS

	ROSANNA MARTINELLI2 T Curriculum
	LIBERATO MARZULLO1 Curriculum
	CARMINE VECCHIONE3 Curriculum

	Objectives
	THIS COURSE IS DESIGNED TO PROVIDE STUDENTS WITH: FUNDAMENTALS OF BIOMOLECULES STRUCTURES AND FUNCTIONS. FUNDAMENTALS OF MAIN METABOLIC PATHWAYS AT THE END OF THE COURSE THE STUDENT WILL ALSO LEARN THE FUNDAMENTAL MOLECULAR MECHANISMS RELATED TO THE PROPAGATION AND MAINTENANCE OF GENETIC INFORMATION; WILL KNOW THE MOLECULAR MECHANISMS OF GENE EXPRESSION AND ITS REGULATIONS; WILL KNOW THE GENERAL PROCESSES THROUGH WHICH GENETIC INFORMATION IS TRANSMITTED AND EXPRESSED. WILL BE ABLE TO DESCRIBE AND SELECT APPROPRIATE TECHNOLOGIES FOR THE ANALYSIS OF BIOMOLECULAR MECHANISMS AND THE IDENTIFICATION OF MOLECULAR ORIGIN OF THE DISEASE. AT THE END OF THE COURSE THE STUDENT WILL HAVE THE ABILITY TO APPLY CORRECTLY THE METHODS DESIGNED TO DETECT CLINICAL FINDING, AND LABORATORY TEST, USING CRITICAL INTERPRETATION AND PATHOPHYSIOLOGICAL CONSIDERATIONS. THEY MUST HAVE ALSO THE CAPACITY TO EVALUATE THE RELATIONSHIP COST / BENEFIT IN THE CHOICE OF DIAGNOSTIC PROCEDURES, TAKING NOTE OF THE PRINCIPLES OF EVIDENCE-BASED MEDICINE. IN AGREEMENT WITH THE DUBLIN DESCRIPTORS, AT THE END OF THE COURSE THE STUDENT WILL DEMONSTRATE: 1.HAVE REACHED A BASIC KNOWLEDGE AND UNDERSTANDING OF BIOCHEMISTRY THAT ALLOWS HIM TO SET UP THE ANALYSIS OF THE CORRELATIONS BETWEEN THE STRUCTURE OF BIOMOLECULES AND THEIR FUNCTIONS IN PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS; 2.ABILITY TO ANALYZE, DISCUSS AND PERFORM SIMPLE CORRELATIONS OF DATA OR ASSUMPTIONS; 3.BEING ABLE EVALUATE INDEPENDENTLY ANY DIFFERENT OPINIONS EXIST IN CERTAIN TOPICS TREATED IN THE COURSE; 4.BEING ABLE TO PROPERLY EXPOSE IN ITALIAN AND WITH PROPERTIES OF LANGUAGE KNOWLEDGE ACQUIRED; 5.KNOWING HOW TO COMMUNICATE WITH THEIR PEERS IN A CRITICAL MANNER THE THEMES STUDIED AND TO SUPPORT INTERDISCIPLINARY ARGUMENTS.

THIS COURSE IS DESIGNED TO PROVIDE STUDENTS WITH: FUNDAMENTALS OF BIOMOLECULES STRUCTURES AND FUNCTIONS. FUNDAMENTALS OF MAIN METABOLIC PATHWAYS
AT THE END OF THE COURSE THE STUDENT WILL ALSO LEARN THE FUNDAMENTAL MOLECULAR MECHANISMS RELATED TO THE PROPAGATION AND MAINTENANCE OF GENETIC INFORMATION; WILL KNOW THE MOLECULAR MECHANISMS OF GENE EXPRESSION AND ITS REGULATIONS; WILL KNOW THE GENERAL PROCESSES THROUGH WHICH GENETIC INFORMATION IS TRANSMITTED AND EXPRESSED. WILL BE ABLE TO DESCRIBE AND SELECT APPROPRIATE TECHNOLOGIES FOR THE ANALYSIS OF BIOMOLECULAR MECHANISMS AND THE IDENTIFICATION OF MOLECULAR ORIGIN OF THE DISEASE.
AT THE END OF THE COURSE THE STUDENT WILL HAVE THE ABILITY TO APPLY CORRECTLY THE METHODS DESIGNED TO DETECT CLINICAL FINDING, AND LABORATORY TEST, USING CRITICAL INTERPRETATION AND PATHOPHYSIOLOGICAL CONSIDERATIONS. THEY MUST HAVE ALSO THE CAPACITY TO EVALUATE THE RELATIONSHIP COST / BENEFIT IN THE CHOICE OF DIAGNOSTIC PROCEDURES, TAKING NOTE OF THE PRINCIPLES OF EVIDENCE-BASED MEDICINE.

IN AGREEMENT WITH THE DUBLIN DESCRIPTORS, AT THE END OF THE COURSE THE STUDENT WILL DEMONSTRATE:
1.HAVE REACHED A BASIC KNOWLEDGE AND UNDERSTANDING OF BIOCHEMISTRY THAT ALLOWS HIM TO SET UP THE ANALYSIS OF THE CORRELATIONS BETWEEN THE STRUCTURE OF BIOMOLECULES AND THEIR FUNCTIONS IN PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS;
2.ABILITY TO ANALYZE, DISCUSS AND PERFORM SIMPLE CORRELATIONS OF DATA OR ASSUMPTIONS;
3.BEING ABLE EVALUATE INDEPENDENTLY ANY DIFFERENT OPINIONS EXIST IN CERTAIN TOPICS TREATED IN THE COURSE;
4.BEING ABLE TO PROPERLY EXPOSE IN ITALIAN AND WITH PROPERTIES OF LANGUAGE KNOWLEDGE ACQUIRED;
5.KNOWING HOW TO COMMUNICATE WITH THEIR PEERS IN A CRITICAL MANNER THE THEMES STUDIED AND TO SUPPORT INTERDISCIPLINARY ARGUMENTS.

	Prerequisites
	CHEMISTRY AND PROPAEDEUTIC BIOCHEMISTRY, APPLIED BIOLOGY AND PHYSICS

	Contents
	AMINOACIDS: STRUCTURE, STEREOCHEMISTRY AND DERIVATIVES. PROTEINS: STRUCTURE AND EVOLUTION. MYOGLOBIN, HEMOGLOBIN, MYOSIN AND ACTIN, COLLAGEN ED ELASTIN. ANTIBODIES. CARBOHYDRATES: STRUCTURE AND DERIVATIVES OF SIMPLE AND COMPLEX SUGARS. LIPIDS: FATTY ACIDS, TAG, STEROIDS, ETC. NUCLEIC ACIDS: STRUCTURE, FUNCTION AND COMPOSITION OF NUCLEOTIDES, DNA, RNA. VITAMINS: STRUCTURE, FUNTIONS AND CLASSIFICATION. NUTRITIONAL REQUIREMENT. BIOLOGICAL MEMBRANES: STRUCTURE, ORGANISATION AND COMPOSITION. ENZYME CATALYSIS: KINETIC AND THERMODYNAMIC REMARKS . MICHAELIS AND ALLOSTERIC ENZYMES. INHIBITION AND REGULATION. METABOLIC PATHWAYS: THERMODYNAMICS AND METABOLIC CONTROL. ATP, NAD, FAD GLUCOSE CATABOLISM: AEROBIC AND ANAEROBIC GLYCOLYSIS, AND ITS CONTROL. METABOLISM OF FRUCTOSE, GALACTOSE, MANNOSE. PENTOSE PHOSPHATE PATHWAY. METABOLISM OF GLYCOGEN, GLUCONEOGENESIS, GLICAEMIA REGULATION. KREBS CYCLE: ACETYL-COA, ENZYMES, REGULATION AND RELATED REACTIONS. ELECTRON TRANSPORT CHAIN AND OXIDATIVE PHOSPHORILATION. ATP PRODUCTION, AEROBIC METABOLISM AND ITS IMPLICATIONS. LIPIDS METABOLISM: DIGESTION, LIPOPROTEINS, LIPID HOMEOSTASIS FATTY ACIDS: BETA-OXIDATION, BIOSYNTHESIS AND METABOLIC CONTROL. KETON BODIES. CHOLESTEROL. METABOLISM OF AMINOACIDS AND PROTEINS. TRANSAMINATION, DEAMINATION, DECARBOXYLATION OF AMINOACIDS. UREA CYCLE. BIOSYNTHESIS AND DEGRADATION OF HEME, BIOGENIC AMINES, NITRIC OXIDE. INTEGRATION E REGULATION OF ENERGETIC METABOLISM. ORGAN METABOLISM. CORI CYCLE, GLUCOSE-ALANINE CYCLE, GLUCOSE TRANSPORTERS. HORMONS AND SIGNAL TRANSDUCTION: ENERGETIC METABOLISM REGULATION, ADENILATE CYCLASE, TYROSIN KINASE RECEPTORS, PHOSPHOINOSITIDE. DISORDERS OF METABOLISM: FASTING, DIABETES, OBESITY. METABOLISM OF NUCLEOTIDES: SYNTHESIS AND DEGRADATION. URIC ACID DESTINY BIOCHEMISTRY OF ORAL CAVITY. CA AND P METABOLISM. BONE, CALCIFICATION AND REABSORPTION. THE ROLE OF COLLAGEN. THE FORMATION OF DENTAL ENAMEL. SALIVA: FUNCTIONS AND CHEMICAL CONSTITUENTS, SALIVARY PROTEINS. CREVICULAR GINGIVAL FLUID: CHEMICAL CONSTITUENTS. BIOCHEMICAL BASES OF CARIES STRUCTURE OF NUCLEIC ACIDS AND THEIR INTERACTIONS WITH PROTEINS: STRUCTURE AND TOPOLOGY OF DNA; STRUCTURE OF RNA, RIBOZYMES, CHROMATIN, HISTONES AND NUCLEOSOMES, REGULATION OF CHROMATIN STRUCTURE REPLICATION AND REPAIR OF DNA DAMAGE: MECHANISM OF ACTION OF DNA POLYMERASE, STAGE OF START AND TERMINATION OF REPLICATION, TELOMERES AND TELOMERASE; REPLICATION ERRORS AND THEIR REPAIR; DIFFERENT TYPES OF MUTATION. TRANSCRIPTION AND POST-TRANSCRIPTIONAL MODIFICATIONS, STRUCTURE AND FUNCTION OF RNA POLYMERASE, PROMOTERS OF RNA POLYMERASE I AND III; PROMOTERS OF RNA POLYMERASE II, GENERAL TRANSCRIPTION FACTORS, THE MEDIATOR COMPLEX; MESSENGER RNA PROCESSING: CAPPING, SPLICING AND POLYADENYLATION; THE SPLICEOSOME, SELF-SPLICING INTRONS, ALTERNATIVE SPLICING. TRANSLATION: OPEN READING FRAME; STRUCTURE AND FUNCTION OF TRNA, AMINOACYL-TRNA SYNTHETASES, THE RIBOSOME, INITIATION, ELONGATION AND TERMINATION OF TRANSLATION, THE GENETIC CODE. REGULATION OF GENE EXPRESSION: TRANSCRIPTIONAL ACTIVATORS; SIGNAL INTEGRATION AND COMBINATORIAL CONTROL, SIGNAL TRANSDUCTION AND CONTROL OF TRANSCRIPTIONAL REGULATORS; GENE SILENCING, EPIGENETICS, RNA INTERFERENCE; SYNTHESIS AND FUNCTION OF MIRNAS RECOMBINANT DNA TECHNOLOGY AND GENE EXPRESSION ANALYSIS: ELECTROPHORESIS OF NUCLEIC ACIDS, RESTRICTION ENZYMES, VECTORS AND CLONING STRATEGIES; EMSA; FOOTPRINTING; SOUTHERN AND NORTHERN BLOT;; PCR, RT-PCR, DNA SEQUENCING, HIGH THROUGHPUT TECHNOLOGIES.

Contents

AMINOACIDS: STRUCTURE, STEREOCHEMISTRY AND DERIVATIVES.
PROTEINS: STRUCTURE AND EVOLUTION. MYOGLOBIN, HEMOGLOBIN, MYOSIN AND ACTIN, COLLAGEN ED ELASTIN. ANTIBODIES.
CARBOHYDRATES: STRUCTURE AND DERIVATIVES OF SIMPLE AND COMPLEX SUGARS.
LIPIDS: FATTY ACIDS, TAG, STEROIDS, ETC.
NUCLEIC ACIDS: STRUCTURE, FUNCTION AND COMPOSITION OF NUCLEOTIDES, DNA, RNA.
VITAMINS: STRUCTURE, FUNTIONS AND CLASSIFICATION. NUTRITIONAL REQUIREMENT.
BIOLOGICAL MEMBRANES: STRUCTURE, ORGANISATION AND COMPOSITION.
ENZYME CATALYSIS: KINETIC AND THERMODYNAMIC REMARKS . MICHAELIS AND ALLOSTERIC ENZYMES. INHIBITION AND REGULATION.
METABOLIC PATHWAYS: THERMODYNAMICS AND METABOLIC CONTROL. ATP, NAD, FAD
GLUCOSE CATABOLISM: AEROBIC AND ANAEROBIC GLYCOLYSIS, AND ITS CONTROL. METABOLISM OF FRUCTOSE, GALACTOSE, MANNOSE. PENTOSE PHOSPHATE PATHWAY.
METABOLISM OF GLYCOGEN, GLUCONEOGENESIS, GLICAEMIA REGULATION.
KREBS CYCLE: ACETYL-COA, ENZYMES, REGULATION AND RELATED REACTIONS.
ELECTRON TRANSPORT CHAIN AND OXIDATIVE PHOSPHORILATION. ATP PRODUCTION, AEROBIC METABOLISM AND ITS IMPLICATIONS.
LIPIDS METABOLISM: DIGESTION, LIPOPROTEINS, LIPID HOMEOSTASIS
FATTY ACIDS: BETA-OXIDATION, BIOSYNTHESIS AND METABOLIC CONTROL. KETON BODIES. CHOLESTEROL.
METABOLISM OF AMINOACIDS AND PROTEINS. TRANSAMINATION, DEAMINATION, DECARBOXYLATION OF AMINOACIDS. UREA CYCLE. BIOSYNTHESIS AND DEGRADATION OF HEME, BIOGENIC AMINES, NITRIC OXIDE.
INTEGRATION E REGULATION OF ENERGETIC METABOLISM. ORGAN METABOLISM. CORI CYCLE, GLUCOSE-ALANINE CYCLE, GLUCOSE TRANSPORTERS. HORMONS AND SIGNAL TRANSDUCTION: ENERGETIC METABOLISM REGULATION, ADENILATE CYCLASE, TYROSIN KINASE RECEPTORS, PHOSPHOINOSITIDE. DISORDERS OF METABOLISM: FASTING, DIABETES, OBESITY.
METABOLISM OF NUCLEOTIDES: SYNTHESIS AND DEGRADATION. URIC ACID DESTINY
BIOCHEMISTRY OF ORAL CAVITY. CA AND P METABOLISM. BONE, CALCIFICATION AND REABSORPTION. THE ROLE OF COLLAGEN. THE FORMATION OF DENTAL ENAMEL. SALIVA: FUNCTIONS AND CHEMICAL CONSTITUENTS, SALIVARY PROTEINS. CREVICULAR GINGIVAL FLUID: CHEMICAL CONSTITUENTS. BIOCHEMICAL BASES OF CARIES
STRUCTURE OF NUCLEIC ACIDS AND THEIR INTERACTIONS WITH PROTEINS: STRUCTURE AND TOPOLOGY OF DNA; STRUCTURE OF RNA, RIBOZYMES, CHROMATIN, HISTONES AND NUCLEOSOMES, REGULATION OF CHROMATIN STRUCTURE
REPLICATION AND REPAIR OF DNA DAMAGE: MECHANISM OF ACTION OF DNA POLYMERASE, STAGE OF START AND TERMINATION OF REPLICATION, TELOMERES AND TELOMERASE; REPLICATION ERRORS AND THEIR REPAIR; DIFFERENT TYPES OF MUTATION.
TRANSCRIPTION AND POST-TRANSCRIPTIONAL MODIFICATIONS, STRUCTURE AND FUNCTION OF RNA POLYMERASE, PROMOTERS OF RNA POLYMERASE I AND III; PROMOTERS OF RNA POLYMERASE II, GENERAL TRANSCRIPTION FACTORS, THE MEDIATOR COMPLEX; MESSENGER RNA PROCESSING: CAPPING, SPLICING AND POLYADENYLATION; THE SPLICEOSOME, SELF-SPLICING INTRONS, ALTERNATIVE SPLICING.
TRANSLATION: OPEN READING FRAME; STRUCTURE AND FUNCTION OF TRNA, AMINOACYL-TRNA SYNTHETASES, THE RIBOSOME, INITIATION, ELONGATION AND TERMINATION OF TRANSLATION, THE GENETIC CODE.
REGULATION OF GENE EXPRESSION: TRANSCRIPTIONAL ACTIVATORS; SIGNAL INTEGRATION AND COMBINATORIAL CONTROL, SIGNAL TRANSDUCTION AND CONTROL OF TRANSCRIPTIONAL REGULATORS; GENE SILENCING, EPIGENETICS, RNA INTERFERENCE; SYNTHESIS AND FUNCTION OF MIRNAS
RECOMBINANT DNA TECHNOLOGY AND GENE EXPRESSION ANALYSIS: ELECTROPHORESIS OF NUCLEIC ACIDS, RESTRICTION ENZYMES, VECTORS AND CLONING STRATEGIES; EMSA; FOOTPRINTING; SOUTHERN AND NORTHERN BLOT;; PCR, RT-PCR, DNA SEQUENCING, HIGH THROUGHPUT TECHNOLOGIES.

	Teaching Methods
	LECTURES

	Verification of learning
	WRITTEN AND ORAL EXAM

	Texts
	BAYNES AND DOMINICZAK, BIOCHIMICA MEDICA, ED. ELSEVIER LEHNINGER, NELSON & COX, PRINCIPI DI BIOCHIMICA, ED. ZANICHELLI MATHEWS, VAN HOLD, AHEREN, BIOCHIMICA, ED. AMBROSIANA STRYER, BIOCHIMICA, ED. ZANICHELLI VOET & VOET, BIOCHIMICA, ED. ZANICHELLI CAMPBELL & FARRELL, BIOCHIMICA, ED. EDISES SILIPRANDI-TETTAMANTI. BIOCHIMICA MEDICA – STRUTTURALE, METABOLICA E FUNZIONALE IV EDIZIONE. PICCIN J.D. WATSON ET AL, BIOLOGIA MOLECOLARE DEL GENE, SESTA EDIZIONE CASA ED. ZANICHELLI LEWIN ET AL., CELLULE, CASA ED. ZANICHELLI ALBERTS ET AL., “BIOLOGIA MOLECOLARE DELLA CELLULA”, V EDIZIONE, CASA ED. ZANICHELLI WEAVER, BIOLOGIA MOLECOLARE, CASA ED. MCGRAW-HILL MEDICINA DI LABORATORIO - LOGICA E PATOLOGIA CLINICA AUTORE: ANTONOZZI - GULLETTA, EDITORE: PICCIN, EDIZIONE: II 2014, VOLUME:UNICO LECTURES SLIDES

Texts

BAYNES AND DOMINICZAK, BIOCHIMICA MEDICA, ED. ELSEVIER
LEHNINGER, NELSON & COX, PRINCIPI DI BIOCHIMICA, ED. ZANICHELLI
MATHEWS, VAN HOLD, AHEREN, BIOCHIMICA, ED. AMBROSIANA
STRYER, BIOCHIMICA, ED. ZANICHELLI
VOET & VOET, BIOCHIMICA, ED. ZANICHELLI
CAMPBELL & FARRELL, BIOCHIMICA, ED. EDISES
SILIPRANDI-TETTAMANTI. BIOCHIMICA MEDICA – STRUTTURALE, METABOLICA E FUNZIONALE
IV EDIZIONE. PICCIN
J.D. WATSON ET AL, BIOLOGIA MOLECOLARE DEL GENE, SESTA EDIZIONE CASA ED. ZANICHELLI
LEWIN ET AL., CELLULE, CASA ED. ZANICHELLI
ALBERTS ET AL., “BIOLOGIA MOLECOLARE DELLA CELLULA”, V EDIZIONE, CASA ED. ZANICHELLI
WEAVER, BIOLOGIA MOLECOLARE, CASA ED. MCGRAW-HILL
MEDICINA DI LABORATORIO - LOGICA E PATOLOGIA CLINICA
AUTORE: ANTONOZZI - GULLETTA, EDITORE: PICCIN, EDIZIONE: II 2014, VOLUME:UNICO
LECTURES SLIDES

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Carmine VECCHIONE | C.I. BIOCHIMICA E BIOLOGIA