Stefano PIOTTO PIOTTO | BIOLOGICAL PHYSICAL CHEMISTRY AND PHARMACEUTICAL MATERIALS

cod. 0760400042

BIOLOGICAL PHYSICAL CHEMISTRY AND PHARMACEUTICAL MATERIALS

	0760400042
	DIPARTIMENTO DI FARMACIA
	EQF7
	PHARMACEUTICAL CHEMISTRY AND TECHNOLOGY
	2024/2025

PERCORSO SHARED STUDY PATHWAY

	YEAR OF COURSE 5
	YEAR OF DIDACTIC SYSTEM 2023
	AUTUMN SEMESTER

TEACHING UNITS

	SSD	CFU	HOURS	ACTIVITY	TYPE OF ACTIVITY
1	CHIMICA FISICA BIOLOGICA MODULO DI CHIMICA FISICA BIOLOGICA E MATERIALI DI INTERESSE FARMACEUTICO
	CHIM/02	3	24	LESSONS	SUPPLEMENTARY COMPULSORY SUBJECTS
2	MATERIALI DI INTERESSE FARMACEUTICO MODULO DI CHIMICA FISICA BIOLOGICA E MATERIALI DI INTERESSE FARMACEUTICO
	CHIM/07	3	24	LESSONS	SUPPLEMENTARY COMPULSORY SUBJECTS

PROFESSORS

	STEFANO PIOTTO PIOTTO2 T Curriculum
	ALESSANDRA FEOLI1 Curriculum

	Objectives
	THE COURSE IS AIMED AT THE ACQUISITION OF KNOWLEDGE AND UNDERSTANDING OF 1) THE CHEMICAL-PHYSICAL CONCEPTS OF THERMODYNAMICS, KINETICS AND SPECTROSCOPY AND THE INVESTIGATIVE TECHNIQUES USED FOR THE EVALUATION OF BIOLOGICAL PROCESSES AND 2) THE FUNDAMENTAL CONCEPTS INVOLVING THE STRUCTURE AND BEHAVIOR OF BOTH SYNTHETIC AND NATURAL POLYMERIC MATERIALS USED IN INDUSTRY WITH AN IN-DEPTH FOCUS ON THE BIOMEDICAL AND PHARMACEUTICAL FIELDS. ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING: THE STUDENT WILL HAVE KNOWLEDGE OF THE RELATIONSHIP BETWEEN THE CHARACTERISTICS OF SYNTHETIC AND NATURALLY OCCURRING POLYMERS AND THEIR PROPERTIES AND APPLICATIONS IN THE PHARMACEUTICAL AND BIOMEDICAL FIELDS. KNOWLEDGE AND UNDERSTANDING SKILLS: THE STUDENT APPLYING THE ACQUIRED SKILLS SHOULD BE ABLE TO USE CHEMICAL PHYSICAL MODELS TO INTERPRET BIOLOGICAL PROCESSES AND LIGAND-BIOMOLECULE INTERACTIONS.

THE COURSE IS AIMED AT THE ACQUISITION OF KNOWLEDGE AND UNDERSTANDING OF 1) THE CHEMICAL-PHYSICAL CONCEPTS OF THERMODYNAMICS, KINETICS AND SPECTROSCOPY AND THE INVESTIGATIVE TECHNIQUES USED FOR THE EVALUATION OF BIOLOGICAL PROCESSES AND 2) THE FUNDAMENTAL CONCEPTS INVOLVING THE STRUCTURE AND BEHAVIOR OF BOTH SYNTHETIC AND NATURAL POLYMERIC MATERIALS USED IN INDUSTRY WITH AN IN-DEPTH FOCUS ON THE BIOMEDICAL AND PHARMACEUTICAL FIELDS.
ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING:
THE STUDENT WILL HAVE KNOWLEDGE OF THE RELATIONSHIP BETWEEN THE CHARACTERISTICS OF SYNTHETIC AND NATURALLY OCCURRING POLYMERS AND THEIR PROPERTIES AND APPLICATIONS IN THE PHARMACEUTICAL AND BIOMEDICAL FIELDS.
KNOWLEDGE AND UNDERSTANDING SKILLS:
THE STUDENT APPLYING THE ACQUIRED SKILLS SHOULD BE ABLE TO USE CHEMICAL PHYSICAL MODELS TO INTERPRET BIOLOGICAL PROCESSES AND LIGAND-BIOMOLECULE INTERACTIONS.

	Prerequisites
	IN ORDER TO UNDERSTAND AND BE ABLE TO APPLY THE TOPICS COVERED IN THE TEACHING, IT IS NECESSARY TO HAVE FULLY ACQUIRED THE OBJECTIVES AND PASSED THE PREPARATORY EXAMS OF THE FOLLOWING COURSES: GENERAL AND INORGANIC CHEMISTRY, ANALYTICAL CHEMISTRY AND PHYSICAL CHEMISTRY; GENERAL BIOCHEMISTRY; AND APPLIED BIOCHEMISTRY.

	Contents
	THE COURSE IS DIVIDED INTO TWO MODULES: BIOLOGICAL PHYSICAL CHEMISTRY MODULE (24 HRS, 3 CFU) AND MATERIALS OF PHARMACEUTICAL INTEREST MODULE (24 HRS, 3 CFU) FOR A TOTAL OF 48 HOURS OF LECTURES (6 CFU). BIOLOGICAL PHYSICAL CHEMISTRY MODULE: MOLECULAR SPECTROSCOPY, FLUORESCENCE-BASED BIOPHYSICAL TECHNIQUES AND THERMODYNAMICS OF DENATURATION. GENERAL FEATURES. ELECTRON SPECTROSCOPY, DECAY OF EXCITED STATES. FLUORESCENCE AND PHOSPHORESCENCE. QUENCHING. RESONANCE ENERGY TRANSFER. UV-VISIBLE SPECTROSCOPY AND LAMBERT BEER'S LAW. FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET), TIME-RESOLVED FLUORESCENCE RESONANCE ENERGY TRANSFER (TR-FRET) AND AMPLIFIED LUMINESCENCE PROXIMITY HOMOGENEOUS ASSAY (ALPHA). THERMAL AND CHEMICAL DENATURATION OF PROTEINS. STABILITY CURVE OF PROTEINS. BIOPHYSICAL TECHNIQUES THAT RELY ON PROTEIN DENATURATION: DIFFERENTIAL SCANNING FLUORIMETRY (DSF) AND NANO-DIFFERENTIAL SCANNING FLUORIMETRY (NANODSF). (12H, 1.5 CFU) BIOPHYSICAL TECHNIQUES USED FOR BINDING STUDIES ON ISOLATED PROTEINS AND IN CELLULAR CONTEXTS. MICROSCALE THERMOPHORESIS (MST). SURFACE PLASMON RESONANCE (SPR). ISOTHERMAL TITRATION CALORIMETRY (ITC). MICROFLUIDIC MOBILITY SHIFT ASSAY (MMSA). LIGAND-OBSERVED NMR. CHEMICAL SHIFT PERTURBATIONS. HSQC. NOESY. STD-NMR. WATER-LOGSY. PRINCIPLES, APPLICATIONS AND DETERMINATION OF AFFINITY, THERMODYNAMIC AND KINETIC PARAMETERS. IN-CELL TARGET ENGAGEMENT. BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET), CELLULAR THERMAL SHIFT ASSAY (CETSA), DRUG AFFINITY RESPONSIVE TARGET STABILITY (DARTS). (12H, 1.5 CFU) MATERIALS OF PHARMACEUTICAL INTEREST MODULE: OVERVIEW OF POLYMERIC MATERIALS. CLASSIFICATION OF POLYMERS. POLYMERS AND TEMPERATURE. COMPOSITION AND MOLECULAR MASSES. SYNTHESIS OF MACROMOLECULES. BIOMATERIALS, CLASSIFICATION. POLYMERIC BIOMATERIALS: POLYAMIDES, POLYESTERS, POLYOLEFINS, POLYURETHANES AND POLYUREAS, POLYACRYLATES, FLUORINATED POLYMERS, BIODEGRADABLE POLYMERS. PHARMACEUTICAL APPLICATIONS OF BIOMATERIALS. (12H, 1.5 CFU) INTRODUCTION TO POLYMERIC MATERIALS CHARACTERIZATION TECHNIQUES: DIFFERENTIAL SCANNING CALORIMETRY (DSC), THERMOGRAVIMETRIC ANALYSIS (TGA), X-RAY (RX), GEL PERMEATION CHROMATOGRAPHY (GPC). MODELING OF POLYMERIC MATERIALS. OVERVIEW OF SIMULATION METHODS. MOLECULAR DYNAMICS. CHEMO-INFORMATICS TECHNIQUES. APPLICATIONS. (12H, 1.5 CFU)

Contents

THE COURSE IS DIVIDED INTO TWO MODULES: BIOLOGICAL PHYSICAL CHEMISTRY MODULE (24 HRS, 3 CFU) AND MATERIALS OF PHARMACEUTICAL INTEREST MODULE (24 HRS, 3 CFU) FOR A TOTAL OF 48 HOURS OF LECTURES (6 CFU).

BIOLOGICAL PHYSICAL CHEMISTRY MODULE:

MOLECULAR SPECTROSCOPY, FLUORESCENCE-BASED BIOPHYSICAL TECHNIQUES AND THERMODYNAMICS OF DENATURATION. GENERAL FEATURES. ELECTRON SPECTROSCOPY, DECAY OF EXCITED STATES. FLUORESCENCE AND PHOSPHORESCENCE. QUENCHING. RESONANCE ENERGY TRANSFER. UV-VISIBLE SPECTROSCOPY AND LAMBERT BEER'S LAW. FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET), TIME-RESOLVED FLUORESCENCE RESONANCE ENERGY TRANSFER (TR-FRET) AND AMPLIFIED LUMINESCENCE PROXIMITY HOMOGENEOUS ASSAY (ALPHA). THERMAL AND CHEMICAL DENATURATION OF PROTEINS. STABILITY CURVE OF PROTEINS. BIOPHYSICAL TECHNIQUES THAT RELY ON PROTEIN DENATURATION: DIFFERENTIAL SCANNING FLUORIMETRY (DSF) AND NANO-DIFFERENTIAL SCANNING FLUORIMETRY (NANODSF). (12H, 1.5 CFU)

BIOPHYSICAL TECHNIQUES USED FOR BINDING STUDIES ON ISOLATED PROTEINS AND IN CELLULAR CONTEXTS. MICROSCALE THERMOPHORESIS (MST). SURFACE PLASMON RESONANCE (SPR). ISOTHERMAL TITRATION CALORIMETRY (ITC). MICROFLUIDIC MOBILITY SHIFT ASSAY (MMSA). LIGAND-OBSERVED NMR. CHEMICAL SHIFT PERTURBATIONS. HSQC. NOESY. STD-NMR. WATER-LOGSY. PRINCIPLES, APPLICATIONS AND DETERMINATION OF AFFINITY, THERMODYNAMIC AND KINETIC PARAMETERS. IN-CELL TARGET ENGAGEMENT. BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET), CELLULAR THERMAL SHIFT ASSAY (CETSA), DRUG AFFINITY RESPONSIVE TARGET STABILITY (DARTS). (12H, 1.5 CFU)

MATERIALS OF PHARMACEUTICAL INTEREST MODULE:

OVERVIEW OF POLYMERIC MATERIALS. CLASSIFICATION OF POLYMERS. POLYMERS AND TEMPERATURE. COMPOSITION AND MOLECULAR MASSES. SYNTHESIS OF MACROMOLECULES. BIOMATERIALS, CLASSIFICATION. POLYMERIC BIOMATERIALS: POLYAMIDES, POLYESTERS, POLYOLEFINS, POLYURETHANES AND POLYUREAS, POLYACRYLATES, FLUORINATED POLYMERS, BIODEGRADABLE POLYMERS. PHARMACEUTICAL APPLICATIONS OF BIOMATERIALS. (12H, 1.5 CFU)

INTRODUCTION TO POLYMERIC MATERIALS CHARACTERIZATION TECHNIQUES: DIFFERENTIAL SCANNING CALORIMETRY (DSC), THERMOGRAVIMETRIC ANALYSIS (TGA), X-RAY (RX), GEL PERMEATION CHROMATOGRAPHY (GPC).
MODELING OF POLYMERIC MATERIALS. OVERVIEW OF SIMULATION METHODS. MOLECULAR DYNAMICS. CHEMO-INFORMATICS TECHNIQUES. APPLICATIONS. (12H, 1.5 CFU)

	Teaching Methods
	TEACHING METHODS: TEACHING INVOLVES FACE-TO-FACE LECTURES FOR A TOTAL OF 48 TOTAL HOURS (6 CFU), WHICH ARE CONDUCTED IN THE CLASSROOM WITH THE AID OF POWERPOINT PROJECTIONS. ATTENDANCE OF CLASSROOM LECTURES IS MANDATORY. IN ORDER TO TAKE THE FINAL EXAM, THE STUDENT MUST HAVE ATTENDED AT LEAST 70% OF THE HOURS COVERED BY THE TEACHING.

	Verification of learning
	FOR THE BIOLOGICAL PHYSICAL CHEMISTRY MODULE, THE EXAMINATION WILL INCLUDE AN ORAL TEST CONSISTING OF AN INTERVIEW WITH QUESTIONS AND DISCUSSION IN ORDER TO ASCERTAIN THE LEVEL OF UNDERSTANDING ACHIEVED BY THE STUDENT ON THE CONTENTS INDICATED IN THE SYLLABUS AND THE STUDENT'S ABILITY TO APPLY THE KNOWLEDGE ACQUIRED. THE ORAL TEST WILL ALSO ALLOW TO VERIFY THE STUDENT'S AUTONOMOUS ORGANIZATION OF EXPOSITION AND EXPOSITORY ABILITY USING APPROPRIATE TERMINOLOGY. THE EXAMINATION IS PASSED WITH A SCORE OF AT LEAST 18/30. FOR THE MATERIALS OF PHARMACEUTICAL INTEREST MODULE, THE EXAMINATION INCLUDES AN ORAL TEST, INCLUDING THE EXPOSITION OF A TECHNICAL PAPER PRODUCED BY THE STUDENT INDEPENDENTLY ON A TOPIC INDICATED BY THE LECTURER. THE TEST IS GRADED IN THIRTIETHS AND IS CONSIDERED PASSED WITH A MINIMUM GRADE OF 18/30. THE ORAL TEST WILL ALSO ALLOW TO VERIFY THE STUDENT'S ABILITY TO COMMUNICATE WITH PROPERTIES OF LANGUAGE AND AUTONOMOUS ORGANIZATION OF THE EXPOSITION ON THE SAME TOPICS WITH THEORETICAL CONTENT. THE FINAL GRADE IS GIVEN BY THE AVERAGE OF THE MARKS GIVEN FOR EACH MODULE.

Verification of learning

FOR THE BIOLOGICAL PHYSICAL CHEMISTRY MODULE, THE EXAMINATION WILL INCLUDE AN ORAL TEST CONSISTING OF AN INTERVIEW WITH QUESTIONS AND DISCUSSION IN ORDER TO ASCERTAIN THE LEVEL OF UNDERSTANDING ACHIEVED BY THE STUDENT ON THE CONTENTS INDICATED IN THE SYLLABUS AND THE STUDENT'S ABILITY TO APPLY THE KNOWLEDGE ACQUIRED.
THE ORAL TEST WILL ALSO ALLOW TO VERIFY THE STUDENT'S AUTONOMOUS ORGANIZATION OF EXPOSITION AND EXPOSITORY ABILITY USING APPROPRIATE TERMINOLOGY. THE EXAMINATION IS PASSED WITH A SCORE OF AT LEAST 18/30.

FOR THE MATERIALS OF PHARMACEUTICAL INTEREST MODULE, THE EXAMINATION INCLUDES AN ORAL TEST, INCLUDING THE EXPOSITION OF A TECHNICAL PAPER PRODUCED BY THE STUDENT INDEPENDENTLY ON A TOPIC INDICATED BY THE LECTURER. THE TEST IS GRADED IN THIRTIETHS AND IS CONSIDERED PASSED WITH A MINIMUM GRADE OF 18/30. THE ORAL TEST WILL ALSO ALLOW TO VERIFY THE STUDENT'S ABILITY TO COMMUNICATE WITH PROPERTIES OF LANGUAGE AND AUTONOMOUS ORGANIZATION OF THE EXPOSITION ON THE SAME TOPICS WITH THEORETICAL CONTENT.

THE FINAL GRADE IS GIVEN BY THE AVERAGE OF THE MARKS GIVEN FOR EACH MODULE.

	Texts
	THE REFERENCE TEXTS FOR THE BIOLOGICAL PHYSICAL CHEMISTRY MODULE ARE AS FOLLOWS: ELEMENTS OF PHYSICAL CHEMISTRY, PETER WILLIAM ATKINS, JULIO DE PAULA (ZANICHELLI) APPLIED BIOPHYSICS FOR DRUG DISCOVERY, EDITOR(S): DONALD HUDDLER, EDWARD R. ZARTLER (WILEY) BIOPHYSICAL TECHNIQUES, IAIN D. CAMPBELL (OXFORD UNIVERSITY PRESS) THE REFERENCE TEXTS FOR THE MATERIALS OF PHARMACEUTICAL INTEREST MODULE ARE AS FOLLOWS: BIOMATERIALS SCIENCE, BUDDY D. RATNER, ALAN S. HOFFMAN (ELSEVIER ACADEMIC PRESS) HANDOUTS PROVIDED DURING THE COURSE.

Texts

THE REFERENCE TEXTS FOR THE BIOLOGICAL PHYSICAL CHEMISTRY MODULE ARE AS FOLLOWS:
ELEMENTS OF PHYSICAL CHEMISTRY, PETER WILLIAM ATKINS, JULIO DE PAULA (ZANICHELLI)
APPLIED BIOPHYSICS FOR DRUG DISCOVERY, EDITOR(S): DONALD HUDDLER, EDWARD R. ZARTLER (WILEY)
BIOPHYSICAL TECHNIQUES, IAIN D. CAMPBELL (OXFORD UNIVERSITY PRESS)

THE REFERENCE TEXTS FOR THE MATERIALS OF PHARMACEUTICAL INTEREST MODULE ARE AS FOLLOWS:
BIOMATERIALS SCIENCE, BUDDY D. RATNER, ALAN S. HOFFMAN (ELSEVIER ACADEMIC PRESS)
HANDOUTS PROVIDED DURING THE COURSE.

	More Information
	ALL COURSE INFORMATION AND LECTURES WILL BE AVAILABLE ON THE COURSE TEAMS CLASS

Lessons Timetable

BETA VERSION Data source ESSE3 [Ultima Sincronizzazione: 2024-11-18]

Stefano PIOTTO PIOTTO | BIOLOGICAL PHYSICAL CHEMISTRY AND PHARMACEUTICAL MATERIALS