Alessandra TOSCO | ENZIMOLOGY
Alessandra TOSCO ENZIMOLOGY
cod. 0760200036
ENZIMOLOGY
| 0760200036 | |
| DIPARTIMENTO DI FARMACIA | |
| EQF7 | |
| PHARMACEUTICAL CHEMISTRY AND TECHNOLOGY | |
| 2016/2017 |
| YEAR OF COURSE 3 | |
| YEAR OF DIDACTIC SYSTEM 2010 | |
| PRIMO SEMESTRE |
| SSD | CFU | HOURS | ACTIVITY | |
|---|---|---|---|---|
| BIO/10 | 5 | 40 | LESSONS |
| Objectives | |
|---|---|
| THE STUDENT AT THE END OF THE COURSE SHOULD BE ABLE TO DEMONSTRATE: KNOWLEDGE AND UNDERSTANDING: ACQUISITION OF KNOWLEDGE OF ENZYMATIC CATALYSIS, KINETIC EQUATIONS, CATALYSIS MECHANISMS AND INHIBITION. FACTORS INFLUENCING CATALYSIS . APPLYING KNOWLEDGE AND UNDERSTANDING: TO BE ABLE TO CRITICALLY DISCUSS AN EXPERIMENTAL PROBLEM OF ENZYMOLOGY, TO FORMULATE HYPOTHESIS AND PROGRAMM EXPERIMENTS TO VERIFY THEM MAKING JUDGEMENTS: TO BE ABLE TO EVALUATE, INTERPRET AND RIELABORATE PUBLISHED DATA IN ENZYMOLOGY FIELD, WITH PARTICULAR REFERENCE TO THEIR IMPACT ON PUBLIC HEALTH AND ENVIRONMENT COMMUNICATION SKILLS: TO BE ABLE TO ELABORATE ENZYMATIC CATALYSIS RESEARCH PROJECTS AND TO ILLUSTRATE THE RESULTS OF THE RESEARCH LEARNING SKILLS: TO BE ABLE TO DEVELOP, DEEPEN AND UPDATE HIS KNOWLEDGE STUDYING SCIENTIFIC PUBLICATIONS AND CONSULTING DATA BASE |
| Prerequisites | |
|---|---|
| IT IS REQUIRED THE ACHIEVEMENT OF THE EDUCATIONAL OBJECTIVES OF THE SUBSEQUENT COURSES: BIOLOGY, ORGANIC CHEMISTRY, BIOCHEMISTRY |
| Contents | |
|---|---|
| ENZYMES DESCRIPTION AND CLASSIFICATION, COENZYMES AND COFACTORS, ISOENZYMES. ENZYMATIC KINETIC: ENZYMATIC CATALYSIS AND REACTION RATE; MICHAELIS-MENTEN EQUATION (EQUILIBRIUM HYPOTHESIS AND STATIONARY STATE HYPOTHESIS); KINETIC PARAMETERS VM, KM, KCAT; MICHAELIS-MENTEN LINEARIZATION; BINDING SITES. ENZYMATIC ACTIVITY INHIBITION: REVERSIBLE AND IRREVERSIBLE. COMPETITIVE INHIBITION AND KI CALCULATION. NON-COMPETITIVE INHIBITION. ACOMPETITIVE AND SUBSTRATE INHIBITION. ENZYMATIC ACTIVITY REGULATION BY COVALENT MODIFICATIONS: REVERSIBLE (PHOSPHORYLATION); IRREVERSIBLE (PARTIAL PROTEOLYSIS): INDUSTRIAL ENZYMES: POTENTIALITY AND APPLICATIONS. IMMOBILIZED ENZYMES. BIOSENSORS. ENZYMES IN DIAGNOSIS. ENZYMATIC ASSAYS: DIRECT, INDIRECT AND DISCONTINUOUS. ENZYMATIC UNITS. LABORATORY: ENZYMATIC ACTIVITY MEASUREMENTS AND INHIBITION. |
| Teaching Methods | |
|---|---|
| LECTURES |
| Verification of learning | |
|---|---|
| ORAL EXAMS |
| Texts | |
|---|---|
| COPELAND, EVALUATION OF ENZYME INHIBITORS IN DRUG DISCOVERY: A GUIDE FOR MEDICAL CHEMISTS AND PHARMACOLOGISTS, WILEY |
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