| DNA AND RNA ANALYSIS: PURIFICATION OF GENOMIC AND PLASMIDIC DNA AND RNA; ANALYSIS BY ELECTROPHORESIS AND BLOTTING. RECOMBINANT VECTORS CONSTRUCTION: BACTERIAL TRANSFORMATION; PLASMIDS; SELECTIVE MARKERS; LAMBDA, M13 AND COSMID VECTORS; ARTIFICIAL CHROMOSOMES: YAC, PAC, BAC AND HAC; RESTRICTION AND MODIFICATION ENZYMES. PRODUCTION OF RECOMBINANT PROTEINS: E. COLI EXPRESSION; EXPRESSION VECTORS; INDUCIBLE AND CONSTITUTIVE PROMOTERS; EXPRESSION IN YEAST (S.CEREVISIAE, P. PASTORIS, S. POMBE), IN INSECT CELLS AND IN HIGHER EUKARYOTES. USAGE OF RECOMBINANT PROTEINS IN RESEARCH AND PHARMACEUTICAL INDUSTRY. PCR AND ITS APPLICATION IN BIOMEDICAL FIELDS: REACTION CONDITIONS; QUANTITATIVE PCR; DIGNOSIS OF GENETIC DISEASES; PATERNITY TEST; FORENSIC APPLICATIONS. DNA SEQUENCING: METHODS OF SEQUENCING; GENOME PROJECTS; HUMAN GENOME PROJECT. CLONING STRATEGIES: GENOMIC DNA AND CDNA LIBRARIES; LIBRARY ANALYSIS; HYBRIDIZATION; CHROMOSOME WALKING AND DIFFERENTIAL ANALYSIS. PROMOTER ANALYSIS: REPORTER PLASMIDS; EMSA; FOOTPRINTING; CHROMATIN IMMUNOPRECIPITATION. PROTEIN-PROTEIN INTERACTIONS: TWO-HYBRID; CO-IMMUNOPRECIPITATION; FISHHING FOR PATNERS; SPR TECHNOLOGY. GENE TRANSFER IN ANIMAL CELLS: CELL COLTURES; TRANSFECTION METHODOLOGIES. ANIMAL GENETIC MODIFICATION: PRONUCLEUS INJECTION; EMBRYONIC STEM CELLS; TRANSGENIC MICE; GENE THERAPY. POST-GENOMIC ANALYSIS: DIFFERENTIAL EXPRESSION ANALYSIS; MICROARRAY; PROTEOMIC ANALYSIS. |
