Anna MARABOTTI | INTERACTOMICS: AN OVERVIEW
Anna MARABOTTI INTERACTOMICS: AN OVERVIEW
cod. 8803300023
INTERACTOMICS: AN OVERVIEW
| 8803300023 | |
| DEPARTMENT OF CHEMISTRY AND BIOLOGY "ADOLFO ZAMBELLI" | |
| P.H.D. COURSE | |
| CHEMICAL, BIOLOGICAL AND ENVIRONMENTAL SCIENCES | |
| 2022/2023 |
| OBBLIGATORIO | |
| YEAR OF COURSE 2 | |
| YEAR OF DIDACTIC SYSTEM 2021 | |
| SPRING SEMESTER |
| SSD | CFU | HOURS | ACTIVITY | |
|---|---|---|---|---|
| BIO/10 | 1 | 8 | LESSONS |
| Objectives | |
|---|---|
| KNOWLEDGE AND UNDERSTANDING THIS TEACHING AIMS TO INTRODUCE THE STUDENT TO THE PROBLEM OF KNOWLEDGE AND UNDERSTANDING OF THE COMPLEX MOLECULAR INTERACTIONS UNDERLYING THE FUNCTIONING OF LIVING ORGANISMS, AND TO THE WAY IN WHICH IT IS POSSIBLE TO STUDY THESE INTERACTIONS WITH DIFFERENT TYPES OF APPROACHES. THE STUDENT WILL THEREFORE UNDERSTAND THAT EVEN THE SIMPLEST ORGANISMS SUCH AS BACTERIA OR CELLS DEPEND, FOR THEIR SURVIVAL, ON THE INTERCHANGE AND RECIPROCAL REGULATION OF THE VARIOUS MOLECULAR ENTITIES, AND WILL KNOW THE TECHNIQUES DEVELOPED FOR THEIR STUDY. ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING AT THE END OF THIS TEACHING, THE STUDENT WILL ACQUIRE BASIC KNOWLEDGE ABOUT THE DIFFERENT WAYS IN WHICH MACROMOLECULES (IN PARTICULAR, PROTEINS) INTERACT WITH EACH OTHER, AND THE TECHNIQUES, BOTH "WET" AND "IN SILICO", DEVELOPED FOR THEIR STUDY, UNDERSTANDING THEIR LIMITATIONS AND THEREFORE WHICH TECHNIQUES ARE MOST APPROPRIATE FOR THE STUDY OF DIFFERENT TYPES OF PHENOMENA OF INTEREST. |
| Prerequisites | |
|---|---|
| THE SUCCESSFUL ATTENDANCE OF TEACHING REQUIRES BASIC KNOWLEDGE OF BIOCHEMISTRY AND BIOCHEMICAL TECHNIQUES FOR THE STUDY OF MACROMOLECULES (IN PARTICULAR, PROTEINS). |
| Contents | |
|---|---|
| THE TEACHING IS DIVIDED INTO LECTURES. THE TOPICS COVERED ARE THE FOLLOWING: - INTRODUCTION TO INTERACTOMICS: HISTORICAL NOTES, MEANING, APPLICATIONS: ABOUT 2H OF FRONTAL LESSONS - MAIN METHODS FOR THE STUDY OF INTERACTOMICS IN VIVO: YEAST-2-HYBRID AND VARIANTS, TANDEM AFFINITY PURIFICATION: ABOUT 2H OF FRONTAL LESSONS - MAIN METHODS FOR THE STUDY OF INTERACTOMICS IN VITRO: CO-IMMUNOPRECIPITATION, PULLDOWN, SPR, CROSSLINKS: ABOUT 2H OF LECTURES - MAIN COMPUTATIONAL METHODS FOR THE PREDICTION AND CATALOGING OF PROTEIN-PROTEIN INTERACTIONS: ABOUT 2 HOURS OF LECTURES. |
| Teaching Methods | |
|---|---|
| THE COURSE INCLUDES 8 HOURS (1 CFU) OF LECTURES, WHICH WILL TAKE PLACE WITH THE HELP OF SLIDE PROJECTIONS AND ACCESS TO MULTIMEDIA MATERIAL FREELY AVAILABLE ONLINE |
| Verification of learning | |
|---|---|
| THE ACHIEVEMENT OF THE TEACHING OBJECTIVES IS CERTIFIED BY PASSING AN EXAM WITH EVALUATION THROUGH A JUDGMENT OF MERIT (INSUFFICIENT, SUFFICIENT, GOOD, EXCELLENT). VERIFICATION OF LEARNING WILL TAKE PLACE THROUGH AN ORAL INTERVIEW THAT WILL FOCUS ON THE TOPICS COVERED IN THE COURSE. |
| Texts | |
|---|---|
| THE TEACHER WILL PROVIDE STUDENTS WITH UPDATED REVIEWS RELATING TO THEORETICAL TOPICS AND SCIENTIFIC ARTICLES WITH PRACTICAL EXAMPLES OF APPLICATION OF THE TECHNIQUES COVERED IN THE COURSE. |
| More Information | |
|---|---|
| REGULAR ATTENDANCE AT LECTURES IS STRONGLY RECOMMENDED. |
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