Maria STRIANESE | ANALYTICAL AND BIOMOLECULAR METHODS
Maria STRIANESE ANALYTICAL AND BIOMOLECULAR METHODS
cod. 0512800031
ANALYTICAL AND BIOMOLECULAR METHODS
| 0512800031 | |
| DIPARTIMENTO DI CHIMICA E BIOLOGIA "ADOLFO ZAMBELLI" | |
| BIOLOGICAL SCIENCES | |
| 2015/2016 |
| OBBLIGATORIO | |
| YEAR OF COURSE 3 | |
| YEAR OF DIDACTIC SYSTEM 2010 | |
| SECONDO SEMESTRE |
| SSD | CFU | HOURS | ACTIVITY | ||
|---|---|---|---|---|---|
| METODOLOGIE BIOMOLECOARI | |||||
| BIO/11 | 5 | 40 | LESSONS | ||
| BIO/11 | 1 | 12 | LAB | ||
| BIO/11 | 0 | 12 | LAB | ||
| METODOLOGIE ANALITICHE | |||||
| CHIM/01 | 4 | 32 | LESSONS | ||
| CHIM/01 | 2 | 24 | LAB | ||
| CHIM/01 | 0 | 48 | LAB | ||
| Objectives | |
|---|---|
| TO GIVE KNOWLEDGE OF THE BASICS OF THE MAJOR CLASSICAL METHODS OF ANALYSIS. TO GIVE KNOWLEDGE ABOUT STRUCTURE-FUNCTION RELATIONSHIP OF BIOLOGICAL MACROMOLECULES AND ABOUT THE MAIN METHODS OF INVESTIGATION IN BIOCHEMISTRY AND MOLECULAR BIOLOGY. TO DEVELOP THE ABILITY TO INTERPRET AND PERFORM AN ANALYTICAL PROTOCOL, AND DESCRIBE THE RESULTS WITH MATHEMATICAL RIGOR. TO STIMULATE THE ABILITY OF UNDERSTAND AND APPLY AN EXPERIMENTAL PROTOCOL CONCERNING HANDLING OF BIOMOLECULES. TO DEVELOP SKILLS TO DISCUSS AND ANALYZE SCIENTIFIC TOPICS. TO DEVELOP THE ABILITY TO DESCRIBE EXPERIMENTAL METHODS TO STUDY BIOMOLECULES. TO DEVELOP A POSITIVE ATTITUDE TO OBTAIN RESULTS. TO DEVELOP A CRITICAL THOUGHT CONCERNING THE IMPORTANCE OF MODERN BIOCHEMICAL METHODS IN BIOLOGICAL AND BIOTECHNOLOGICAL FIELDS. |
| Prerequisites | |
|---|---|
| IT IS REQUIRED SKILLS IN GENERAL CHEMISTRY, ORGANIC CHEMISTRY, MATHEMATICS, PHYSICS AND BIOCHEMISTRY. |
| Contents | |
|---|---|
| STOICHIOMETRY AND BASIC OPERATION OF ANALYTICAL CHEMISTRY. MAJOR PHASES OF CHEMICAL ANALYSIS. ERRORS IN ANALYTICAL DETERMINATIONS. THE ACID-BASE EQUILIBRIA IN CHEMICAL ANALYSIS. APPLICATIONS OF ACID-BASE REACTIONS QUANTITATIVE AND QUALITATIVE ANALYSIS. APPLICATIONS OF COMPLEXATION REACTIONS QUANTITATIVE AND QUALITATIVE ANALYSIS. THE REDOX BALANCE CHEMICAL ANALYSIS. APPLICATION OF REDOX REACTIONS QUANTITATIVE AND QUALITATIVE ANALYSIS. THE CHEMICAL ANALYSIS SOLUBILITY EQUILIBRIA. APPLICATIONS OF PRECIPITATION REACTIONS QUANTITATIVE AND QUALITATIVE ANALYSIS. CLASSIFICATION OF METHODS OF INSTRUMENTAL ANALYSIS. SAMPLING TECHNIQUES AND PRETREATMENT. SPECTROPHOTOMETRIC MEASUREMENTS. ELECTROCHEMICAL METHODS. CHROMATOGRAPHY. METHODS OF HOMOGENIZATION OF CELLS AND TISSUES. ELECTROPHORESIS OF PROTEINS AND NUCLEIC ACIDS. METHODS OF BLOTTING. CENTRIFUGATION. ELEMENTS OF IMMUNOLOGICAL METHODS. PCR. ELEMENTS OF MASS SPECTROMETRY. |
| Teaching Methods | |
|---|---|
| LECTURES IN THE CLASSROOM WITH PROJECTION OF TEACHING MATERIALS IN ELECTRONIC FORM, MADE AVAILABLE ON THE WEB. EXERCISES IN CLASS. LABORATORY PRACTISES. |
| Verification of learning | |
|---|---|
| ORAL EXAMINATION INTEGRATED BY A WRITTEN TEST. THE PROOF IS IN A SURVEY OF ORAL QUESTIONS REGARDING THE PROGRAM. |
| Texts | |
|---|---|
| D.S. HAGE, J.D. CARR, CHIMICA ANALITICA ED ANALISI QUANTITATIVA, PICCIN, PADOVA, 2012. A. PELOSO, PROBLEMI DI CHIMICA GENERALE, CORTINA, PADOVA, 2006; D. C. HARRIS, CHIMICA ANALITICA QUANTITATIVA, ZANICHELLI, BOLOGNA; SKOOG D. A, HOLLER, F. J., CROUCH, S. R., CHIMICA ANALITICA STRUMENTALE, 2A ED. EDISES 2009 REED, HOLMES, WEYERS E JONES, METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI, ZANICHELLI |
| More Information | |
|---|---|
| METHOD OF FREQUENCY. STRONGLY RECOMMENDED THE FREQUENCY TO THE COURSE. |
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