2. Professors
  3. TOSCO Alessandra
  4. Teaching

MICROBIOLOGY AND MOLECULAR BIOLOGY

Alessandra TOSCO MICROBIOLOGY AND MOLECULAR BIOLOGY

0760100019
DIPARTIMENTO DI FARMACIA
CORSO DI LAUREA MAGISTRALE A CICLO UNICO DI 5 ANNI
PHARMACY
2015/2016

OBBLIGATORIO
YEAR OF COURSE 3
YEAR OF DIDACTIC SYSTEM 2010
SECONDO SEMESTRE
CFUHOURSACTIVITY
1MICROBIOLOGIA
540LESSONS
2PRINCIPI E TECNICHE DI BIOLOGIA MOLECOLARE
540LESSONS
AMALIA PORTA1 T Curriculum
ALESSANDRA TOSCO2 Curriculum
Objectives
THE STUDENT AT THE END OF THE COURSE SHOULD BE ABLE TO DEMONSTRATE:
KNOWLEDGE AND UNDERSTANDING:
ACQUISITION OF KNOWLEDGE ON BIOCHEMICAL PROPERTIES OF INFORMATIONAL MOLECULES, DNA AND RNA, FROM A STRUCTURAL AND A FUNCTIONAL POINT OF VIEW
UNDERSTAND THE MAIN MOLECULAR MECHANISMS INVOLVED IN MICROBIAL VIRULENCE;

APPLYING KNOWLEDGE AND UNDERSTANDING:
TO BE ABLE TO APPLY THE KNOWLEDGE ON MOLECULAR PROCESSES ON DUPLICATION, TRANSCRIPTION AND TRANSLATION TO THE UNDERSTANDING OF THE MOLECULAR MECHANISMS OF DRUGS RELATED THESE PROCESSES.BE ABLE TO APPLY THE KNOWLEDGE OF THE MAIN MECHANISMS OF BACTERIAL, FUNGAL AND PARASITIC VIRULENCE TO LIMIT THE ONSET AND SPREADING OF HUMAN INFECTIOUS DISEASES;

MAKING JUDGEMENTS:
TO ABLE TO INTEGRATE HIS KNOWLEDGE ON MOLECULAR PROCESSES OF THE CELL WITH BIOCHEMICAL AND PHARMACOLOGICAL STUDIES.TO ANALYZE AND INTEGRATE CRITICALLY DIFFERENT CONCEPTS OF BACTERIOLOGY, MYCOLOGY AND PARASITOLOGY,

COMMUNICATION SKILLS:
HAVE THE ABILITY TO TRANSFER THE ACQUIRED KNOWLEDGE ALSO TO INDIVIDUALS NOT BELONGING TO HEALTH CARE SYSTEM, UTILIZING IT THEORETICALLY AND PRACTICALLY (IN LABORATORY ANALYSES)

LEARNING SKILLS:
TO BE ABLE TO UPDATE HIS KNOWLEDGE THROUGH SCIENTIFIC PUBLICATIONS ON MOLECULAR BIOLOGY AND MICROBIOLOGY.
Prerequisites
CHEMISTRY, BIOLOGY, BIOCHEMISTRY
Contents
MICROBIOLOGY: -PHYLETIC ORIGIN OF BACTERIA: BACTERIA, ARCHEOBACTERIA AND EUKARYOTES
-KOCH’S POSTULATES
-CELL THEORY
-DIFFERENCES BETWEEN PROKARYOTES AND EUKARYOTES
-BACTERIAL MORPHOLOGY
-BACTERIAL CAPSULE, CELL WALL, FLAGELLA, PILI
-MICROBIAL STAINING METHODS, GRAM STAIN
-MICROBIAL GROWTH, SPORULATION
-BACTERIAL TOXINS: LPS, EXOTOXINS AND THEIR MECHANISMS OF ACTION
-CELL CULTURE
-HUMAN MICROBIOTA

BACTERIA
CLASSIFICATION, STRUCTURE, PHYSIOLOGY, PATHOGENESIS AND VIRULENCE FACTORS OF
ENTEROBACTERIACEE, SALMONELLA AND ANTIGENIC SWITCH OF SALMONELLA;
PSEUDOMONAS;
STAPPHYLOCOCCUS;
STREPTOCOCCUS;
BACILLACEAE, BACILLUS ANTHRACIS, CLOSTRIDIUM TETANI, C. BOTULINUM, C. PERFRINGENS, C. DIFFICILE;
HAEMOPHILUS;
H. PYLORI.

MYCOLOGY
YEAST, MYCELIUM, MYCOSIS, YEAST TO HYPHA TRANSITION
DIMORPHIC FUNGI: H. CAPSULATUM, B. DERMATITIDIS, P. BRASILIENSIS, C. IMMITIS, C. ALBICANS
PATHOGENIC FUNGI C. NEOFORMANS, ASPERGILLUS

VIRUS
MORPHOLOGY, SYMMETRIES; VIRAL GENOMES; BALTIMORE’S CLASSIFICATION; VIRAL REPLICATIVE CYCLES; PATHOGENESIS OF VIRAL INFECTIONS; HUMAN HERPES VIRUSES.

MOLECULAR BIOLOGY:

NUCLEIC ACID STRUCTURE: NUCLEOSIDES E NUCLEOTIDES; WATSON AND CRICK AND THE DOUBLE HELIX STRUCTURE OF DNA; RNA AND ITS STRUCTURES. CHROMATIN: HISTONES AND NUCLEOSOMES; HIGH ORDER CHROMATIN STRUCTURES; NUCLEOSOMES REMODELLING AND HISTONE MODIFICATIONS. DNA REPLICATION: THE MECHANISM OF ACTION OF DNA POLYMERASE; THE ENZYMOLOGY OF REPLICATION; DNA HELICASES, SSB, TOPOISOMERASES AND DNA SLIDING CLAMP; CONTROL OF THE BEGINNING; THE PROBLEM OF TERMINATION: TELOMERS AND TELOMERASES. DNA MUTABILITY AND REPAIR: DNA DAMAGE; REPLICATION ERRORS AND CHEMICAL DAMAGE; REPAIR SYSTEMS; HOMOLOGOUS RECOMBINATION: THE HOLLIDAY MODEL; DSB REPAIR AND CROSSING-OVER; SITE SPECIFIC RECOMBINATION AND TRANSPOSITION. TRANSCRIPTION: RNA POLYMERASE AND TRANSCRIPTION CYCLE; PROKARYOTES; PROMOTER AND SIGMA FACTOR; RHO- DEPENDENT AND RHO-INDEPENDENT TERMINATION. EUKARYOTES; GENERAL FACTORS, MEDIATOR COMPLEX AND TRANSCRIPTIONAL ACTIVATORS; MRNA MATURATION: CAPPING AND POLYADENILATION. SPLICING: SPLICING REACTIONS; SPLICEOSOME MACHINERY; ALTERNATIVE SPLICING; EXON RESHUFFLING. TRANSLATION: TRNA; AMINOACYL-TRNA SYNTHETASE; RIBOSOME STRUCTURE. CONTROL OF THE BEGINNING; THE MECHANISM OF ELONGATION; THE TERMINATION; THE GENETIC CODE. GENE EXPRESSION REGULATION: THE CONTROL OF THE TRANSCRIPTION; TRANSCRIPTION FACTORS; EPIGENETIC REGULATION AND IMPRINTING; POST-TRANSCRIPTIONAL REGULATION; RNA EDITING; RNA INTERFERENCE. FOLDING OF NEW SYNTHESIZED PROTEINS AND POST-TRANSLATIONAL MODIFICATIONS: CHAPERON ASSISTED FOLDING; PROTEIN UBIQUITINILATION AND PROTEASOME COMPLEX.
Teaching Methods
LECTURES AND LABORATORY
Verification of learning
MICROBIOLOGY: MULTIPLE CHOICE TESTS AND ORAL EXAMINATION
Texts
MICROBIOLOGY: PRINCIPI DI MICROBIOLOGIA MEDICA II, G. ANTONELLI, M. CLEMENTI, G. POZZI, G.M. ROSSOLINI. CASA EDITRICE AMBROSIANA
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