Alessandra TOSCO | MICROBIOLOGY AND MOLECULAR BIOLOGY

cod. 0760100019

MICROBIOLOGY AND MOLECULAR BIOLOGY

	0760100019
	DIPARTIMENTO DI FARMACIA
	EQF7
	PHARMACY
	2020/2021

PERCORSO COMUNE Cohort 2018/2019

	OBBLIGATORIO
	YEAR OF COURSE 3
	YEAR OF DIDACTIC SYSTEM 2016
	SECONDO SEMESTRE

TEACHING UNITS

	SSD	CFU	HOURS	ACTIVITY	TYPE OF ACTIVITY
1	MICROBIOLOGIA MODULO DI MICROBIOLOGIA E BIOLOGIA MOLECOLARE
	BIO/19	6	48	LESSONS	BASIC COMPULSORY SUBJECTS
2	BIOLOGIA MOLECOLARE MODULO DI MICROBIOLOGIA E BIOLOGIA MOLECOLARE
	BIO/11	5	40	LESSONS	COMPULSORY SUBJECTS, CHARACTERISTIC OF THE CLASS

PROFESSORS

	AMALIA PORTA1 T Curriculum
	ALESSANDRA TOSCO2 Curriculum

	Objectives
	THE TEACHING IS DIRECTED TO THE ACQUISITION OF KNOWLEDGE AND TO THE COMPREHENSION OF CLASSIFICATION, MORPHOLOGY AND VIRULENCE FACTORS OF THE MAIN MICROORGANISMS AND VIRUSES; OTHER THAN THE MOLECULAR BASES OF DUPLICATION, GENE TRANSCRIPTION AND PROTEIN SYNTHESIS PROCESSES. APPLYING THE ACQUIRED SKILLS, THE STUDENT SHOULD BE ABLE TO ANALYSE AND UNDERTAKE PROBLEMS RELATED TO INFECTIVE PATHOLOGIES CAUSED BY BACTERIA, FUNGI AND VIRUSES IN HUMANS, OTHER THAN PROBLEMS RELATED TO MOLECULAR MECHANISMS OF GENE EXPRESSION, IN PHYSIOLOGIC AND PATHOLOGIC CONDITIONS

THE TEACHING IS DIRECTED TO THE ACQUISITION OF KNOWLEDGE AND TO THE COMPREHENSION OF CLASSIFICATION, MORPHOLOGY AND VIRULENCE FACTORS OF THE MAIN MICROORGANISMS AND VIRUSES; OTHER THAN THE MOLECULAR BASES OF DUPLICATION, GENE TRANSCRIPTION AND PROTEIN SYNTHESIS PROCESSES.
APPLYING THE ACQUIRED SKILLS, THE STUDENT SHOULD BE ABLE TO ANALYSE AND UNDERTAKE PROBLEMS RELATED TO INFECTIVE PATHOLOGIES CAUSED BY BACTERIA, FUNGI AND VIRUSES IN HUMANS, OTHER THAN PROBLEMS RELATED TO MOLECULAR MECHANISMS OF GENE EXPRESSION, IN PHYSIOLOGIC AND PATHOLOGIC CONDITIONS

	Prerequisites
	CHEMISTRY, BIOLOGY, BIOCHEMISTRY

	Contents
	MICROBIOLOGY: -PHYLETIC ORIGIN OF BACTERIA: BACTERIA, ARCHEOBACTERIA AND EUKARYOTES -KOCH’S POSTULATES -CELL THEORY -DIFFERENCES BETWEEN PROKARYOTES AND EUKARYOTES -BACTERIAL MORPHOLOGY -BACTERIAL CAPSULE, CELL WALL, FLAGELLA, PILI -MICROBIAL STAINING METHODS, GRAM STAIN -MICROBIAL GROWTH, SPORULATION -BACTERIAL TOXINS: LPS, EXOTOXINS AND THEIR MECHANISMS OF ACTION -CELL CULTURE -HUMAN MICROBIOTA BACTERIA CLASSIFICATION, STRUCTURE, PHYSIOLOGY, PATHOGENESIS AND VIRULENCE FACTORS OF ENTEROBACTERIACEE, E. COLI, SALMONELLA AND ANTIGENIC SWITCH OF SALMONELLA; HELICOBACTER PYLORI; PSEUDOMONAS; STAPPHYLOCOCCUS; STREPTOCOCCUS; BACILLACEAE, BACILLUS ANTHRACIS, CLOSTRIDIUM TETANI, C. BOTULINUM, C. PERFRINGENS, C. DIFFICILE; FLUCTUATION TEST OPERONS: LAC, CONJUGATION, TRANSFECTION, TRANSFORMATION MYCOLOGY YEAST, MYCELIUM, MYCOSIS, YEAST TO HYPHA TRANSITION DIMORPHIC FUNGI: C. ALBICANS PATHOGENIC FUNGI C. NEOFORMANS, ASPERGILLUS VIRUS MORPHOLOGY, SYMMETRIES; VIRAL GENOMES; BALTIMORE’S CLASSIFICATION; VIRAL REPLICATIVE CYCLES; PATHOGENESIS OF VIRAL INFECTIONS; HUMAN HERPES VIRUSES. BACTERIOPHAGE MOLECULAR BIOLOGY: NUCLEIC ACID STRUCTURE: NUCLEOSIDES E NUCLEOTIDES; WATSON AND CRICK AND THE DOUBLE HELIX STRUCTURE OF DNA; RNA AND ITS STRUCTURES. CHROMATIN: HISTONES AND NUCLEOSOMES; HIGH ORDER CHROMATIN STRUCTURES; NUCLEOSOMES REMODELLING AND HISTONE MODIFICATIONS. DNA REPLICATION: THE MECHANISM OF ACTION OF DNA POLYMERASE; THE ENZYMOLOGY OF REPLICATION; DNA HELICASES, SSB, TOPOISOMERASES AND DNA SLIDING CLAMP; CONTROL OF THE BEGINNING; THE PROBLEM OF TERMINATION: TELOMERS AND TELOMERASES. DNA MUTABILITY AND REPAIR: DNA DAMAGE; REPLICATION ERRORS AND CHEMICAL DAMAGE; REPAIR SYSTEMS; HOMOLOGOUS RECOMBINATION: THE HOLLIDAY MODEL; DSB REPAIR AND CROSSING-OVER; SITE SPECIFIC RECOMBINATION AND TRANSPOSITION. TRANSCRIPTION: RNA POLYMERASE AND TRANSCRIPTION CYCLE; PROKARYOTES; PROMOTER AND SIGMA FACTOR; RHO- DEPENDENT AND RHO-INDEPENDENT TERMINATION. EUKARYOTES; GENERAL FACTORS, MEDIATOR COMPLEX AND TRANSCRIPTIONAL ACTIVATORS; MRNA MATURATION: CAPPING AND POLYADENILATION. SPLICING: SPLICING REACTIONS; SPLICEOSOME MACHINERY; ALTERNATIVE SPLICING; EXON RESHUFFLING. TRANSLATION: TRNA; AMINOACYL-TRNA SYNTHETASE; RIBOSOME STRUCTURE. CONTROL OF THE BEGINNING; THE MECHANISM OF ELONGATION; THE TERMINATION; THE GENETIC CODE. GENE EXPRESSION REGULATION: THE CONTROL OF THE TRANSCRIPTION; TRANSCRIPTION FACTORS; EPIGENETIC REGULATION AND IMPRINTING; POST-TRANSCRIPTIONAL REGULATION; RNA EDITING; RNA INTERFERENCE. FOLDING OF NEW SYNTHESIZED PROTEINS AND POST-TRANSLATIONAL MODIFICATIONS: CHAPERON ASSISTED FOLDING; PROTEIN UBIQUITINILATION AND PROTEASOME COMPLEX.

Contents

MICROBIOLOGY: -PHYLETIC ORIGIN OF BACTERIA: BACTERIA, ARCHEOBACTERIA AND EUKARYOTES
-KOCH’S POSTULATES
-CELL THEORY
-DIFFERENCES BETWEEN PROKARYOTES AND EUKARYOTES
-BACTERIAL MORPHOLOGY
-BACTERIAL CAPSULE, CELL WALL, FLAGELLA, PILI
-MICROBIAL STAINING METHODS, GRAM STAIN
-MICROBIAL GROWTH, SPORULATION
-BACTERIAL TOXINS: LPS, EXOTOXINS AND THEIR MECHANISMS OF ACTION
-CELL CULTURE
-HUMAN MICROBIOTA

BACTERIA
CLASSIFICATION, STRUCTURE, PHYSIOLOGY, PATHOGENESIS AND VIRULENCE FACTORS OF
ENTEROBACTERIACEE, E. COLI, SALMONELLA AND ANTIGENIC SWITCH OF SALMONELLA;
HELICOBACTER PYLORI;
PSEUDOMONAS;
STAPPHYLOCOCCUS;
STREPTOCOCCUS;
BACILLACEAE, BACILLUS ANTHRACIS, CLOSTRIDIUM TETANI, C. BOTULINUM, C. PERFRINGENS, C. DIFFICILE;

FLUCTUATION TEST
OPERONS: LAC,
CONJUGATION, TRANSFECTION, TRANSFORMATION

MYCOLOGY
YEAST, MYCELIUM, MYCOSIS, YEAST TO HYPHA TRANSITION
DIMORPHIC FUNGI: C. ALBICANS
PATHOGENIC FUNGI C. NEOFORMANS, ASPERGILLUS

VIRUS
MORPHOLOGY, SYMMETRIES; VIRAL GENOMES; BALTIMORE’S CLASSIFICATION; VIRAL REPLICATIVE CYCLES; PATHOGENESIS OF VIRAL INFECTIONS; HUMAN HERPES VIRUSES. BACTERIOPHAGE

MOLECULAR BIOLOGY:

NUCLEIC ACID STRUCTURE: NUCLEOSIDES E NUCLEOTIDES; WATSON AND CRICK AND THE DOUBLE HELIX STRUCTURE OF DNA; RNA AND ITS STRUCTURES. CHROMATIN: HISTONES AND NUCLEOSOMES; HIGH ORDER CHROMATIN STRUCTURES; NUCLEOSOMES REMODELLING AND HISTONE MODIFICATIONS. DNA REPLICATION: THE MECHANISM OF ACTION OF DNA POLYMERASE; THE ENZYMOLOGY OF REPLICATION; DNA HELICASES, SSB, TOPOISOMERASES AND DNA SLIDING CLAMP; CONTROL OF THE BEGINNING; THE PROBLEM OF TERMINATION: TELOMERS AND TELOMERASES. DNA MUTABILITY AND REPAIR: DNA DAMAGE; REPLICATION ERRORS AND CHEMICAL DAMAGE; REPAIR SYSTEMS; HOMOLOGOUS RECOMBINATION: THE HOLLIDAY MODEL; DSB REPAIR AND CROSSING-OVER; SITE SPECIFIC RECOMBINATION AND TRANSPOSITION. TRANSCRIPTION: RNA POLYMERASE AND TRANSCRIPTION CYCLE; PROKARYOTES; PROMOTER AND SIGMA FACTOR; RHO- DEPENDENT AND RHO-INDEPENDENT TERMINATION. EUKARYOTES; GENERAL FACTORS, MEDIATOR COMPLEX AND TRANSCRIPTIONAL ACTIVATORS; MRNA MATURATION: CAPPING AND POLYADENILATION. SPLICING: SPLICING REACTIONS; SPLICEOSOME MACHINERY; ALTERNATIVE SPLICING; EXON RESHUFFLING. TRANSLATION: TRNA; AMINOACYL-TRNA SYNTHETASE; RIBOSOME STRUCTURE. CONTROL OF THE BEGINNING; THE MECHANISM OF ELONGATION; THE TERMINATION; THE GENETIC CODE. GENE EXPRESSION REGULATION: THE CONTROL OF THE TRANSCRIPTION; TRANSCRIPTION FACTORS; EPIGENETIC REGULATION AND IMPRINTING; POST-TRANSCRIPTIONAL REGULATION; RNA EDITING; RNA INTERFERENCE. FOLDING OF NEW SYNTHESIZED PROTEINS AND POST-TRANSLATIONAL MODIFICATIONS: CHAPERON ASSISTED FOLDING; PROTEIN UBIQUITINILATION AND PROTEASOME COMPLEX.

	Teaching Methods
	LECTURES

	Verification of learning
	ORAL EXAMINATION

	Texts
	PRINCIPI DI MICROBIOLOGIA MEDICA; ANTONELLI ET AL.- CASA EDITRICE AMBROSIANA. MICROBIOLOGIA MOLECOLARE E CELLULARE, MARESCA ET AL. MCGRAW-HILL MOLECULAR BIOLOGY: J.D. WATSON ET AL. – MOLECULAR BIOLOGY OF THE GENE, VII EDIZIONE – ZANICHELLI ISBN 88-08-36480-7 G. CAPRANICO, E. MARTEGANI, G. MUSCI, G. RAUGEI, T. RUSSO, N. ZAMBRANO, V. ZAPPAVIGNA - BIOLOGIA MOLECOLARE - EDISES

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	LECTURES IN ITALIAN, LOCATION DEPT. OF PHARMACY UD: MICROBIOLOGY (PROF. AMALIA PORTA) UD: MOLECULAR BIOLOGY (PROF. ALESSANDRA TOSCO)

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Alessandra TOSCO | MICROBIOLOGY AND MOLECULAR BIOLOGY